Reference - Detail
|Author||Rosa A, Vlassaks E, Pichaud F, Baum B.|
|Title||Ect2/Pbl acts via Rho and polarity proteins to direct the assembly of an isotropic actomyosin cortex upon mitotic entry.|
Entry into mitosis is accompanied by profound changes in cortical actomyosin organization. Here, we delineate a pathway downstream of the RhoGEF Pbl/Ect2 that directs this process in a model epithelium. Our data suggest that the release of Pbl/Ect2 from the nucleus at mitotic entry drives Rho-dependent activation of Myosin-II and, in parallel, induces a switch from Arp2/3 to Diaphanous-mediated cortical actin nucleation that depends on Cdc42, aPKC, and Par6. At the same time, the mitotic relocalization of these apical protein complexes to more lateral cell surfaces enables Cdc42/aPKC/Par6 to take on a mitosis-specific function-aiding the assembly of a relatively isotropic metaphase cortex. Together, these data reveal how the repolarization and remodeling of the actomyosin cortex are coordinated upon entry into mitosis to provide cells with the isotropic and rigid form they need to undergo faithful chromosome segregation and division in a crowded tissue environment.
|MeSH||Actin Cytoskeleton / metabolism Actin-Related Protein 2-3 Complex / metabolism* Actomyosin / metabolism* Animals Blotting, Western Cell Nucleus / metabolism Cells, Cultured Drosophila Proteins / metabolism* Drosophila melanogaster / genetics Drosophila melanogaster / growth & development Drosophila melanogaster / metabolism* Epithelial Cells / cytology Epithelial Cells / metabolism Fluorescent Antibody Technique GTP-Binding Proteins / metabolism* Guanine Nucleotide Exchange Factors / metabolism* Immunoprecipitation Mitosis / physiology* Protein Binding Protein Kinase C / metabolism*|
|WOS Category||DEVELOPMENTAL BIOLOGY CELL BIOLOGY|