RRC ID 33139
Author Sugahara R, Mon H, Lee JM, Shiotsuki T, Kusakabe T.
Title Differential contribution of the Fanconi anemia-related proteins to repair of several types of DNA damage in cultured silkworm cells.
Journal FEBS Lett
Abstract The silkworm Fanconi anemia (FA) pathway is required for normal cellular resistance to mitomycin C (MMC) in silkworms, but little is known about the requirement for repair of other types of DNA damage. Here we report that silkworm cells deficient for FA proteins FancD2 and FancM exhibit normal sensitivities to hydroxyurea (HU) and camptothecin (CPT), although FancM-dependent FancD2 monoubiquitination is induced upon these treatments. Similar results were observed in cells depleted for Rmi1 and Mhf1, which interact with the FancM protein. We also found that Rad51-knockdown cells exhibited normal sensitivity to HU despite induction of double-strand breaks by HU treatment.
Volume 588(21)
Pages 3959-63
Published 2014-11-3
DOI 10.1016/j.febslet.2014.09.009
PII S0014-5793(14)00684-X
PMID 25240201
MeSH Animals Bombyx / cytology* Camptothecin / pharmacology Cells, Cultured DNA Damage* DNA Helicases / deficiency DNA Helicases / metabolism* DNA Repair* Fanconi Anemia Complementation Group D2 Protein / deficiency Fanconi Anemia Complementation Group D2 Protein / metabolism* Histones / metabolism Homologous Recombination / drug effects Humans Hydroxyurea / pharmacology Mitomycin / pharmacology Phosphorylation / drug effects Ubiquitination / drug effects
IF 3.057
Times Cited 1