| RRC ID |
33163
|
| 著者 |
Kawasaki K, Yamada S, Ogata K, Saito Y, Takahama A, Yamada T, Matsumoto K, Kose H.
|
| タイトル |
Use of Drosophila as an evaluation method reveals imp as a candidate gene for type 2 diabetes in rat locus Niddm22.
|
| ジャーナル |
J Diabetes Res
|
| Abstract |
Type 2 diabetes (T2D) is one of the most common human diseases. QTL analysis of the diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) rats has identified numerous hyperglycemic loci. However, molecular characterization and/or gene identification largely remains to be elucidated due mostly to the weak genetic variances contributed by each locus. Here we utilized Drosophila melanogaster as a secondary model organism for functional evaluation of the candidate gene. We demonstrate that the tissue specific knockdown of a homologue of igf2bp2 RNA binding protein leads to increased sugar levels similar to that found in the OLETF rat. In the mutant, the expression of two of the insulin-like peptides encoded in the fly genome, dilp2 and dilp3, were found to be downregulated. Consistent with previous reports of dilp mutants, the imp mutant flies exhibited an extension of life span; in contrast, starvation tolerance was reduced. These results further reinforce the possibility that imp is involved in sugar metabolism by modulating insulin expression.
|
| 巻・号 |
2015
|
| ページ |
758564
|
| 公開日 |
2015-3-2
|
| DOI |
10.1155/2015/758564
|
| PMID |
25821834
|
| PMC |
PMC4363715
|
| MeSH |
Animals
Blood Glucose / metabolism
Body Weight
Diabetes Mellitus, Type 2 / genetics*
Diabetes Mellitus, Type 2 / metabolism*
Disease Models, Animal
Drosophila Proteins / metabolism
Drosophila melanogaster
Female
Gene Expression Profiling
Green Fluorescent Proteins / metabolism
Hyperglycemia / metabolism
Insulin / metabolism
Insulin-Like Growth Factor Binding Protein 2 / metabolism
Male
Polymorphism, Genetic
RNA Interference
Rats
Rats, Inbred OLETF
|
| IF |
2.965
|
| 引用数 |
1
|
| リソース情報 |
| ショウジョウバエ |
Imp protein trap strain (DGRC#110921) |
