RRC ID |
33203
|
Author |
Gawroński P, Ariyadasa R, Himmelbach A, Poursarebani N, Kilian B, Stein N, Steuernagel B, Hensel G, Kumlehn J, Sehgal SK, Gill BS, Gould P, Hall A, Schnurbusch T.
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Title |
A distorted circadian clock causes early flowering and temperature-dependent variation in spike development in the Eps-3Am mutant of einkorn wheat.
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Journal |
Genetics
|
Abstract |
Viable circadian clocks help organisms to synchronize their development with daily and seasonal changes, thereby providing both evolutionary fitness and advantage from an agricultural perspective. A high-resolution mapping approach combined with mutant analysis revealed a cereal ortholog of Arabidopsis thaliana LUX ARRHYTHMO/PHYTOCLOCK 1 (LUX/PCL1) as a promising candidate for the earliness per se 3 (Eps-3A(m)) locus in einkorn wheat (Triticum monococcum L.). Using delayed fluorescence measurements it was shown that Eps-3A(m) containing einkorn wheat accession KT3-5 had a distorted circadian clock. The hypothesis was subsequently confirmed by performing a time course study on central and output circadian clock genes, which showed arrhythmic transcript patterns in KT3-5 under constant ambient conditions, i.e., constant light and temperature. It was also demonstrated that variation in spikelet number between wild-type and mutants is sensitive to temperature, becoming negligible at 25°. These observations lead us to propose that the distorted clock is causative for both early flowering and variation in spike size and spikelet number, and that having a dysfunctional LUX could have neutral, or even positive, effects in warmer climates. To test the latter hypothesis we ascertained sequence variation of LUX in a range of wheat germplasm. We observed a higher variation in the LUX sequence among accessions coming from the warmer climate and a unique in-frame mutation in early-flowering Chinese T. turgidum cultivar 'Tsing Hua no. 559.' Our results emphasize the importance of the circadian clock in temperate cereals as a promising target for adaptation to new environments.
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Volume |
196(4)
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Pages |
1253-61
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Published |
2014-4-1
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DOI |
10.1534/genetics.113.158444
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PII |
genetics.113.158444
|
PMID |
24443443
|
PMC |
PMC3982698
|
MeSH |
Chromosome Mapping
Circadian Clocks*
DNA Mutational Analysis
Flowers / genetics
Flowers / growth & development*
Genetic Variation
Mutation
Plant Proteins / genetics*
Plant Proteins / metabolism
Temperature
Triticum / classification
Triticum / genetics
Triticum / growth & development*
|
IF |
4.015
|
Times Cited |
41
|
WOS Category
|
GENETICS & HEREDITY
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Resource |
Wheat |
T. monococcum: KT003-001
KT003-005 |