論文 - 詳細
| RRC ID | 33260 |
|---|---|
| 著者 | Fukaya T, Iwakawa HO, Tomari Y. |
| タイトル | MicroRNAs block assembly of eIF4F translation initiation complex in Drosophila. |
| ジャーナル | Mol Cell |
| Abstract |
miRNAs silence their complementary target mRNAs by translational repression as well as by poly(A) shortening and mRNA decay. In Drosophila, miRNAs are typically incorporated into Argonaute1 (Ago1) to form the effector complex called RNA-induced silencing complex (RISC). Ago1-RISC associates with a scaffold protein GW182, which recruits additional silencing factors. We have previously shown that miRNAs repress translation initiation by blocking formation of the 48S and 80S ribosomal complexes. However, it remains unclear how ribosome recruitment is impeded. Here, we examined the assembly of translation initiation factors on the target mRNA under repression. We show that Ago1-RISC induces dissociation of eIF4A, a DEAD-box RNA helicase, from the target mRNA without affecting 5' cap recognition by eIF4E in a manner independent of GW182. In contrast, direct tethering of GW182 promotes dissociation of both eIF4E and eIF4A. We propose that miRNAs act to block the assembly of the eIF4F complex during translation initiation. |
| 巻・号 | 56(1) |
| ページ | 67-78 |
| 公開日 | 2014-10-2 |
| DOI | 10.1016/j.molcel.2014.09.004 |
| PII | S1097-2765(14)00711-4 |
| PMID | 25280104 |
| MeSH | Animals Argonaute Proteins / metabolism Argonaute Proteins / physiology Cells, Cultured Drosophila Drosophila Proteins / metabolism Drosophila Proteins / physiology Eukaryotic Initiation Factor-4A / metabolism Eukaryotic Initiation Factor-4E / metabolism Eukaryotic Initiation Factor-4F / metabolism* MicroRNAs / genetics MicroRNAs / metabolism MicroRNAs / physiology* Models, Genetic* Peptide Chain Initiation, Translational* RNA, Messenger / metabolism RNA-Induced Silencing Complex / metabolism |
| IF | 15.584 |
| 引用数 | 62 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pENTR-eIF4G (RDB13216) |