RRC ID 33331
Author Saito Y, Takeda J, Adachi K, Nobe Y, Kobayashi J, Hirota K, Oliveira DV, Taoka M, Isobe T.
Title RNase MRP cleaves pre-tRNASer-Met in the tRNA maturation pathway.
Journal PLoS One
Abstract Ribonuclease mitochondrial RNA processing (RNase MRP) is a multifunctional ribonucleoprotein (RNP) complex that is involved in the maturation of various types of RNA including ribosomal RNA. RNase MRP consists of a potential catalytic RNA and several protein components, all of which are required for cell viability. We show here that the temperature-sensitive mutant of rmp1, the gene for a unique protein component of RNase MRP, accumulates the dimeric tRNA precursor, pre-tRNA(Ser-Met). To examine whether RNase MRP mediates tRNA maturation, we purified the RNase MRP holoenzyme from the fission yeast Schizosaccharomyces pombe and found that the enzyme directly and selectively cleaves pre-tRNA(Ser-Met), suggesting that RNase MRP participates in the maturation of specific tRNA in vivo. In addition, mass spectrometry-based ribonucleoproteomic analysis demonstrated that this RNase MRP consists of one RNA molecule and 11 protein components, including a previously unknown component Rpl701. Notably, limited nucleolysis of RNase MRP generated an active catalytic core consisting of partial mrp1 RNA fragments, which constitute "Domain 1" in the secondary structure of RNase MRP, and 8 proteins. Thus, the present study provides new insight into the structure and function of RNase MRP.
Volume 9(11)
Pages e112488
Published 2014-1-1
DOI 10.1371/journal.pone.0112488
PII PONE-D-13-36098
PMID 25401760
PMC PMC4234475
MeSH Amino Acid Sequence Amino Acid Substitution Base Sequence Catalysis Endoribonucleases / chemistry Endoribonucleases / genetics Endoribonucleases / metabolism* Enzyme Activation Mutation Nucleic Acid Conformation RNA Precursors / genetics* RNA Precursors / metabolism* RNA, Transfer, Ser / genetics* RNA, Transfer, Ser / metabolism* Schizosaccharomyces / genetics Schizosaccharomyces / metabolism
IF 2.74
Times Cited 6
DNA material pFOX1-CHATA (RDB08052) pCtrlki-kanMX6 (RDB08041) pCtHATAki-kanMX6 (RDB08051) pCtFEM3ki-kanMX6 (RDB08042) pCtFLATAki-kanMX6 (RDB08045) pFOX1-CFLATA (RDB08048) pCtFEM3ki-rpr2-kanMX6T (RDB08044) pCtrlki-spac25b8.16-kanMX6T (RDB08046) pCtFLATAki-spac25b8.16-kanMX6T (RDB08047) pFOX1-spac323.08(+)-FLATA (RDB08049) pCtFEM3ki-spac323.08-kanMX6T (RDB08043) pCtFLATAki-Pspac323.08-SalI-spac323.08(+)-kanMX6T (RDB08050) pFOX1-rpl701-HATA (RDB08053) pFOX1-spcc16c4.05-HATA (RDB08054) pFOX1-spcc16c4.19-HATA (RDB08055)