論文 - 詳細
| RRC ID | 33351 |
|---|---|
| 著者 | Jones KL, Keasling JD. |
| タイトル | Construction and characterization of F plasmid-based expression vectors. |
| ジャーナル | Biotechnol Bioeng |
| Abstract |
A low-copy expression vector has been constructed from a 9 Kbp region of the Escherichia coli F plasmid containing the oriV and oriS origins of replication. This plasmid carries the beta-lactamase gene (Apr) and the araBAD promoter/araC regulator for arabinose-inducible gene expression. A derivative which carries a lacZ reporter gene was found to be stably maintained for at least 150 generations. A related multi-copy plasmid was stably maintained in arabinose-free medium, but no plasmid-bearing segregants remained after 60 generations when lacZ expression was induced. Induced expression resulted in 27% (multi-copy) and 12% (low-copy) decreases in growth rate. The uninduced levels of beta-galactosidase were 200 units (multi-copy) and 15 units (low-copy). |
| 巻・号 | 59(6) |
| ページ | 659-65 |
| 公開日 | 1998-9-20 |
| PII | 10.1002/(SICI)1097-0290(19980920)59:6<659::AID-BIT1>3.0.CO;2-E |
| PMID | 10099385 |
| MeSH | Arabinose / metabolism Blotting, Southern Cell Division Cloning, Molecular / methods* Escherichia coli / physiology Genetic Vectors / metabolism* Models, Genetic Plasmids / metabolism* Time Factors beta-Galactosidase / metabolism |
| IF | 4.002 |
| 引用数 | 30 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | pKLJ10 pKLJ12 |