| RRC ID |
33351
|
| 著者 |
Jones KL, Keasling JD.
|
| タイトル |
Construction and characterization of F plasmid-based expression vectors.
|
| ジャーナル |
Biotechnol Bioeng
|
| Abstract |
A low-copy expression vector has been constructed from a 9 Kbp region of the Escherichia coli F plasmid containing the oriV and oriS origins of replication. This plasmid carries the beta-lactamase gene (Apr) and the araBAD promoter/araC regulator for arabinose-inducible gene expression. A derivative which carries a lacZ reporter gene was found to be stably maintained for at least 150 generations. A related multi-copy plasmid was stably maintained in arabinose-free medium, but no plasmid-bearing segregants remained after 60 generations when lacZ expression was induced. Induced expression resulted in 27% (multi-copy) and 12% (low-copy) decreases in growth rate. The uninduced levels of beta-galactosidase were 200 units (multi-copy) and 15 units (low-copy).
|
| 巻・号 |
59(6)
|
| ページ |
659-65
|
| 公開日 |
1998-9-20
|
| PII |
10.1002/(SICI)1097-0290(19980920)59:6<659::AID-BIT1>3.0.CO;2-E
|
| PMID |
10099385
|
| MeSH |
Arabinose / metabolism
Blotting, Southern
Cell Division
Cloning, Molecular / methods*
Escherichia coli / physiology
Genetic Vectors / metabolism*
Models, Genetic
Plasmids / metabolism*
Time Factors
beta-Galactosidase / metabolism
|
| IF |
4.002
|
| 引用数 |
30
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| 原核生物(大腸菌) |
pKLJ10
pKLJ12 |
