RRC ID 33351
著者 Jones KL, Keasling JD.
タイトル Construction and characterization of F plasmid-based expression vectors.
ジャーナル Biotechnol Bioeng
Abstract A low-copy expression vector has been constructed from a 9 Kbp region of the Escherichia coli F plasmid containing the oriV and oriS origins of replication. This plasmid carries the beta-lactamase gene (Apr) and the araBAD promoter/araC regulator for arabinose-inducible gene expression. A derivative which carries a lacZ reporter gene was found to be stably maintained for at least 150 generations. A related multi-copy plasmid was stably maintained in arabinose-free medium, but no plasmid-bearing segregants remained after 60 generations when lacZ expression was induced. Induced expression resulted in 27% (multi-copy) and 12% (low-copy) decreases in growth rate. The uninduced levels of beta-galactosidase were 200 units (multi-copy) and 15 units (low-copy).
巻・号 59(6)
ページ 659-65
公開日 1998-9-20
PII 10.1002/(SICI)1097-0290(19980920)59:6<659::AID-BIT1>3.0.CO;2-E
PMID 10099385
MeSH Arabinose / metabolism Blotting, Southern Cell Division Cloning, Molecular / methods* Escherichia coli / physiology Genetic Vectors / metabolism* Models, Genetic Plasmids / metabolism* Time Factors beta-Galactosidase / metabolism
IF 4.002
引用数 30
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
リソース情報
原核生物(大腸菌) pKLJ10 pKLJ12