論文 - 詳細
| RRC ID | 33548 |
|---|---|
| 著者 | Fukushima T, Tanabe T, Yamamoto H, Hosoya S, Sato T, Yoshikawa H, Sekiguchi J. |
| タイトル | Characterization of a polysaccharide deacetylase gene homologue (pdaB) on sporulation of Bacillus subtilis. |
| ジャーナル | J Biochem |
| Abstract |
The predicted amino acid sequence of Bacillus subtilis ybaN (renamed pdaB) exhibits high similarity to those of several polysaccharide deacetylases. Northern hybridization analysis with sporulation sigma mutants indicated that the pdaB gene is transcribed by EsigmaE RNA polymerase and negatively regulated by SpoIIID. The pdaB mutant was deficient in spore formation. Phase- and electron microscopic observation showed morphological changes of spores in late sporulation periods. The pdaB spores that had lost their viability were empty. Moreover, GFP driven by the promoter of the sspE gene was localized in the forespore compartment for the wild type, but was localized in both the mother cell and forespore compartments for phase-gray/dark forespores of the pdaB mutant. This indicates that GFP expressed in the forespores of the mutant leaks into the mother cells. Therefore, PdaB is necessary to maintain spores after the late stage of sporulation. |
| 巻・号 | 136(3) |
| ページ | 283-91 |
| 公開日 | 2004-9-1 |
| DOI | 10.1093/jb/mvh151 |
| PII | 136/3/283 |
| PMID | 15598884 |
| MeSH | Amidohydrolases / genetics* Amidohydrolases / metabolism Bacillus subtilis / genetics* Bacillus subtilis / metabolism* Bacterial Proteins / chemistry Base Sequence Blotting, Northern Cell Survival Centrifugation, Density Gradient DNA Primers / chemistry DNA-Binding Proteins / chemistry DNA-Directed RNA Polymerases / chemistry Escherichia coli / metabolism Genetic Complementation Test Genotype Green Fluorescent Proteins / metabolism Microscopy, Electron Microscopy, Phase-Contrast Molecular Sequence Data Mutation Picolinic Acids / chemistry Plasmids / metabolism Polysaccharides / chemistry RNA, Messenger / metabolism Sequence Homology, Amino Acid Spores, Bacterial / metabolism* Time Factors Transcription Factors / chemistry |
| IF | 2.476 |
| 引用数 | 18 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(枯草菌) | MBS56 MBS57 MBS58 |