RRC ID 33615
Author Almeida AD, Boije H, Chow RW, He J, Tham J, Suzuki SC, Harris WA.
Title Spectrum of Fates: a new approach to the study of the developing zebrafish retina.
Journal Development
Abstract The ability to image cells live and in situ as they proliferate and differentiate has proved to be an invaluable asset to biologists investigating developmental processes. Here, we describe a Spectrum of Fates approach that allows the identification of all the major neuronal subtypes in the zebrafish retina simultaneously. Spectrum of Fates is based on the combinatorial expression of differently coloured fluorescent proteins driven by the promoters of transcription factors that are expressed in overlapping subsets of retinal neurons. Here, we show how a Spectrum of Fates approach can be used to assess various aspects of neural development, such as developmental waves of differentiation, neuropil development, lineage tracing and hierarchies of fates in the developing zebrafish retina.
Volume 141(9)
Pages 1971-80
Published 2014-5
DOI 10.1242/dev.104760
PII dev.104760
PMID 24718991
PMC PMC3994774
MeSH Animals Animals, Genetically Modified Cell Differentiation Cell Lineage Cell Proliferation Cells, Cultured Clone Cells Fluorescence Genetic Techniques* Luminescent Proteins / metabolism* Retina / cytology Retina / embryology* Zebrafish / embryology* Zebrafish / genetics*
IF 5.763
Times Cited 9
WOS Category DEVELOPMENTAL BIOLOGY
Resource
Zebrafish Tg(chx10:GFP) Tg(vsx2:EGFP)