RRC ID 33623
著者 Braubach OR, Miyasaka N, Koide T, Yoshihara Y, Croll RP, Fine A.
タイトル Experience-dependent versus experience-independent postembryonic development of distinct groups of zebrafish olfactory glomeruli.
ジャーナル J Neurosci
Abstract Olfactory glomeruli are innervated with great precision by the axons of different olfactory sensory neuron types and act as functional units in odor information processing. Approximately 140 glomeruli are present in each olfactory bulb of adult zebrafish; these units consist of either highly stereotypic large glomeruli or smaller anatomically indistinguishable glomeruli. In the present study, we investigated developmental differences among these types of glomeruli. We observed that 10 large and individually identifiable glomeruli already developed before hatching, at 72 h after fertilization, in configurations that resembled their mature organization. However, the cross-sectional area of these glomeruli increased throughout larval development, and they eventually comprised the largest units in postlarval olfactory bulbs. In contrast, small and anatomically indistinguishable glomeruli formed only after hatching, apparently by segregating from five larger precursors that were identifiable during embryonic development. The differentiation of these small glomeruli proceeded with conspicuous variation in number and arrangement, both among larvae and between olfactory bulbs of the same individuals. To determine factors that might contribute to this variability, we investigated the effects of olfactory enrichment on the development of amino acid-responsive lateral glomeruli, which include both large and small units. Larvae reared in an amino acid-enriched environment had normal large lateral glomeruli, but the small lateral glomeruli were more numerous and displayed reduced cross-sectional areas compared with glomeruli in control animals. Our results suggest that large and small glomeruli mature via distinct developmental processes that may be differentially influenced by sensory experience.
巻・号 33(16)
ページ 6905-16
公開日 2013-4-17
DOI 10.1523/JNEUROSCI.5185-12.2013
PII 33/16/6905
PMID 23595749
PMC PMC6618874
MeSH Amino Acids / pharmacology Animals Animals, Genetically Modified Embryo, Nonmammalian GTP-Binding Proteins / metabolism Gene Expression Regulation, Developmental / genetics Gene Expression Regulation, Developmental / physiology* Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Hemocyanins / metabolism Larva Membrane Proteins / metabolism Microscopy, Confocal Nerve Net / drug effects Nerve Net / physiology* Nerve Tissue Proteins / metabolism Neural Pathways / embryology Neural Pathways / growth & development Olfactory Bulb* / cytology Olfactory Bulb* / embryology Olfactory Bulb* / growth & development Olfactory Receptor Neurons / drug effects Olfactory Receptor Neurons / physiology* Zebrafish Zebrafish Proteins / genetics Zebrafish Proteins / metabolism
IF 5.674
引用数 9
WOS 分野 NEUROSCIENCES
リソース情報
ゼブラフィッシュ SAGFF27A Tg(UAS:GFP)