RRC ID 33655
Author Amo R, Fredes F, Kinoshita M, Aoki R, Aizawa H, Agetsuma M, Aoki T, Shiraki T, Kakinuma H, Matsuda M, Yamazaki M, Takahoko M, Tsuboi T, Higashijima S, Miyasaka N, Koide T, Yabuki Y, Yoshihara Y, Fukai T, Okamoto H.
Title The habenulo-raphe serotonergic circuit encodes an aversive expectation value essential for adaptive active avoidance of danger.
Journal Neuron
Abstract Anticipation of danger at first elicits panic in animals, but later it helps them to avoid the real threat adaptively. In zebrafish, as fish experience more and more danger, neurons in the ventral habenula (vHb) showed tonic increase in the activity to the presented cue and activated serotonergic neurons in the median raphe (MR). This neuronal activity could represent the expectation of a dangerous outcome and be used for comparison with a real outcome when the fish is learning how to escape from a dangerous to a safer environment. Indeed, inhibiting synaptic transmission from vHb to MR impaired adaptive avoidance learning, while panic behavior induced by classical fear conditioning remained intact. Furthermore, artificially triggering this negative outcome expectation signal by optogenetic stimulation of vHb neurons evoked place avoidance behavior. Thus, vHb-MR circuit is essential for representing the level of expected danger and behavioral programming to adaptively avoid potential hazard.
Volume 84(5)
Pages 1034-48
Published 2014-12-3
DOI 10.1016/j.neuron.2014.10.035
PII S0896-6273(14)00955-6
PMID 25467985
MeSH 5,7-Dihydroxytryptamine / metabolism Action Potentials / physiology Adaptation, Psychological / physiology Animals Animals, Genetically Modified Avoidance Learning / physiology* Conditioning, Classical / physiology Cues Fear / physiology Habenula / cytology Habenula / physiology* Luminescent Proteins / genetics Luminescent Proteins / metabolism Male Neural Pathways / physiology* Neurotransmitter Agents / metabolism Raphe Nuclei / cytology Raphe Nuclei / physiology* Serotonergic Neurons / physiology* Serotonin / metabolism Transcription Factors / genetics Transcription Factors / metabolism Vesicular Glutamate Transport Protein 2 / genetics Vesicular Glutamate Transport Protein 2 / metabolism Zebrafish Zebrafish Proteins / genetics Zebrafish Proteins / metabolism
IF 14.415
Times Cited 71
Zebrafish Tg(UAS:GFP-TeNT)/rw0146a Tg(UAS:hChR2-mCherry)/rw0147a Tg(dao:GAL4VP16)/rw0148a Tg(dao:Cre-mCherry)/rw0149a Tg(ppp1r14ab:GAL4VP16)/rw0150a Tg(UAS:GFP)