RRC ID 33669
著者 Brustein E, Côté S, Ghislain J, Drapeau P.
タイトル Spontaneous glycine-induced calcium transients in spinal cord progenitors promote neurogenesis.
ジャーナル Dev Neurobiol
Abstract Glycine and GABA are depolarizing during early development, but the purpose of this paradoxical chloride-mediated depolarization remains unclear, especially at early stages. It was previously reported that suppressing glycine signaling from the beginning of development in zebrafish embryos caused an abnormal maintenance of the progenitor population and a specific reduction of spinal interneurons but not of other cell populations. Here, we show that cells including progenitors in the embryonic spinal cord had occasional spontaneous, glycine-mediated calcium transients that were blocked by the glycine antagonist strychnine and the L-type calcium channel blocker nifedipine. As shown previously for chronic block by strychnine, block of these transients by nifedipine reduced interneuron differentiation. Our results indicate that glycinergic depolarization of neural progenitors evokes spontaneous calcium transients that may enhance the interneuron neurogenic program.
巻・号 73(2)
ページ 168-75
公開日 2013-2-1
DOI 10.1002/dneu.22050
PMID 22888055
MeSH Animals Calcium Channel Blockers / pharmacology Calcium Signaling / drug effects* Cell Differentiation / drug effects Cell Differentiation / physiology Data Interpretation, Statistical Embryo, Nonmammalian Glycine / pharmacology* Glycine Agents / pharmacology Green Fluorescent Proteins Immunohistochemistry Interneurons / physiology Microscopy, Confocal Neural Stem Cells / drug effects* Neurogenesis / drug effects* Nifedipine / pharmacology Spinal Cord / cytology Spinal Cord / drug effects* Spinal Cord / growth & development* Strychnine / pharmacology Zebrafish
IF 3.935
引用数 14
WOS 分野 DEVELOPMENTAL BIOLOGY NEUROSCIENCES
リソース情報
ゼブラフィッシュ Tg(chx10:GFP)/nns1