RRC ID 33679
著者 Sittaramane V, Pan X, Glasco DM, Huang P, Gurung S, Bock A, Li S, Wang H, Kawakami K, Matise MP, Chandrasekhar A.
タイトル The PCP protein Vangl2 regulates migration of hindbrain motor neurons by acting in floor plate cells, and independently of cilia function.
ジャーナル Dev Biol
Abstract Vangl2, a core component of the Planar Cell Polarity pathway, is necessary for the caudal migration of Facial Branchiomotor (FBM) neurons in the vertebrate hindbrain. Studies in zebrafish suggest that vangl2 functions largely non-cell autonomously to regulate FBM neuron migration out of rhombomere 4 (r4), but the cell-type within which it acts is not known. Here, we demonstrate that vangl2 functions largely in floor plate cells to regulate caudal neuronal migration. Furthermore, FBM neurons fail to migrate caudally in the mouse Gli2 mutant that lacks the floor plate, suggesting an evolutionarily conserved role for this cell type in neuronal migration. Although hindbrain floor plate cilia are disorganized in vangl2 mutant embryos, cilia appear to be dispensable for neuronal migration. Notably, Vangl2 is enriched in the basolateral, but not apical, membranes of floor plate cells. Taken together, our data suggest strongly that Vangl2 regulates FBM neuron migration by acting in floor plate cells, independently of cilia function.
巻・号 382(2)
ページ 400-12
公開日 2013-10-15
DOI 10.1016/j.ydbio.2013.08.017
PII S0012-1606(13)00442-9
PMID 23988578
PMC PMC3864009
MeSH Animals Cell Movement Cell Polarity Cilia / physiology* Gene Expression Regulation, Developmental* Kruppel-Like Transcription Factors / genetics Kruppel-Like Transcription Factors / metabolism Membrane Proteins / genetics* Membrane Proteins / metabolism Mice Motor Neurons / metabolism* Neurogenesis Rhombencephalon / cytology Rhombencephalon / metabolism* Zebrafish / embryology Zebrafish / metabolism Zebrafish Proteins / genetics* Zebrafish Proteins / metabolism Zinc Finger Protein Gli2
IF 2.896
引用数 15
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
ゼブラフィッシュ Tg(isl1:GFP)/rw0