Reference - Detail
| RRC ID | 3373 |
|---|---|
| Author | Arkblad EL, Tuck S, Pestov NB, Dmitriev RI, Kostina MB, Stenvall J, Tranberg M, Rydström J. |
| Title | A Caenorhabditis elegans mutant lacking functional nicotinamide nucleotide transhydrogenase displays increased sensitivity to oxidative stress. |
| Journal | Free Radic Biol Med |
| Abstract |
Proton-translocating mitochondrial nicotinamide nucleotide transhydrogenase (NNT) was investigated regarding its physiological role in Caenorhabditis elegans. NNT catalyzes the reduction of NADP(+) by NADH driven by the electrochemical proton gradient, Deltap, and is thus a potentially important source of mitochondrial NADPH. Mitochondrial detoxification of reactive oxygen species (ROS) by glutathione-dependent peroxidases depends on NADPH for regeneration of reduced glutathione. Transhydrogenase may therefore be directly involved in the defense against oxidative stress. nnt-1 deletion mutants of C. elegans, nnt-1(sv34), were isolated and shown to grow essentially as wild type under normal laboratory conditions, but with a strongly lowered GSH/GSSG ratio. Under conditions of oxidative stress, caused by the superoxide-generating agent methyl viologen, growth of worms lacking nnt-1 activity was severely impaired. A similar result was obtained by using RNAi. Reintroducing nnt-1 in the nnt-1(sv34) knockout mutant led to a partial rescue of growth under oxidative stress conditions. These results provide evidence for the first time that nnt-1 is important in the defense against mitochondrial oxidative stress. |
| Volume | 38(11) |
| Pages | 1518-25 |
| Published | 2005-6-1 |
| DOI | 10.1016/j.freeradbiomed.2005.02.012 |
| PII | S0891-5849(05)00084-5 |
| PMID | 15890626 |
| MeSH | Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / physiology Cell Proliferation Electrochemistry Gene Deletion Glutathione Green Fluorescent Proteins / metabolism Immunoblotting Mitochondria / metabolism Models, Chemical Models, Genetic Mutation* NADP / chemistry NADP Transhydrogenases / genetics* NADP Transhydrogenases / physiology Oxidative Stress Paraquat / pharmacology Phenotype Plasmids / metabolism Protons RNA Interference RNA, Double-Stranded / chemistry Time Factors |
| IF | 6.17 |
| Times Cited | 77 |
| WOS Category | ENDOCRINOLOGY & METABOLISM BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm358 |