RRC ID 3394
著者 Ou G, Blacque OE, Snow JJ, Leroux MR, Scholey JM.
タイトル Functional coordination of intraflagellar transport motors.
ジャーナル Nature
Abstract Cilia have diverse roles in motility and sensory reception, and defects in cilia function contribute to ciliary diseases such as Bardet-Biedl syndrome (BBS). Intraflagellar transport (IFT) motors assemble and maintain cilia by transporting ciliary precursors, bound to protein complexes called IFT particles, from the base of the cilium to their site of incorporation at the distal tip. In Caenorhabditis elegans, this is accomplished by two IFT motors, kinesin-II and osmotic avoidance defective (OSM)-3 kinesin, which cooperate to form two sequential anterograde IFT pathways that build distinct parts of cilia. By observing the movement of fluorescent IFT motors and IFT particles along the cilia of numerous ciliary mutants, we identified three genes whose protein products mediate the functional coordination of these motors. The BBS proteins BBS-7 and BBS-8 are required to stabilize complexes of IFT particles containing both of the IFT motors, because IFT particles in bbs-7 and bbs-8 mutants break down into two subcomplexes, IFT-A and IFT-B, which are moved separately by kinesin-II and OSM-3 kinesin, respectively. A conserved ciliary protein, DYF-1, is specifically required for OSM-3 kinesin to dock onto and move IFT particles, because OSM-3 kinesin is inactive and intact IFT particles are moved by kinesin-II alone in dyf-1 mutants. These findings implicate BBS ciliary disease proteins and an OSM-3 kinesin activator in the formation of two IFT pathways that build functional cilia.
巻・号 436(7050)
ページ 583-7
公開日 2005-7-28
DOI 10.1038/nature03818
PII nature03818
PMID 16049494
MeSH Animals Biological Transport Caenorhabditis elegans / genetics Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Calcium-Binding Proteins / metabolism Cilia / genetics Cilia / physiology* Flagella / physiology* Kinesins / metabolism Molecular Motor Proteins / genetics Molecular Motor Proteins / physiology* Muscle Proteins / metabolism Mutation / genetics Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism Receptors, Cell Surface / genetics Receptors, Cell Surface / metabolism Time Factors
IF 42.779
引用数 266
WOS 分野 CELL BIOLOGY
リソース情報
線虫 tm324