Reference - Detail
|Author||Phillips CM, Wong C, Bhalla N, Carlton PM, Weiser P, Meneely PM, Dernburg AF.|
|Title||HIM-8 binds to the X chromosome pairing center and mediates chromosome-specific meiotic synapsis.|
The him-8 gene is essential for proper meiotic segregation of the X chromosomes in C. elegans. Here we show that loss of him-8 function causes profound X chromosome-specific defects in homolog pairing and synapsis. him-8 encodes a C2H2 zinc-finger protein that is expressed during meiosis and concentrates at a site on the X chromosome known as the meiotic pairing center (PC). A role for HIM-8 in PC function is supported by genetic interactions between PC lesions and him-8 mutations. HIM-8 bound chromosome sites associate with the nuclear envelope (NE) throughout meiotic prophase. Surprisingly, a point mutation in him-8 that retains both chromosome binding and NE localization fails to stabilize pairing or promote synapsis. These observations indicate that stabilization of homolog pairing is an active process in which the tethering of chromosome sites to the NE may be necessary but is not sufficient.
|MeSH||Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / physiology Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Cell Cycle Proteins / physiology* Chromosome Pairing / genetics Chromosome Pairing / physiology* Disorders of Sex Development / genetics Female In Situ Hybridization, Fluorescence Lamins / analysis Male Meiosis / genetics Meiosis / physiology* Meiotic Prophase I / genetics Microscopy, Fluorescence Mutation / genetics Nuclear Envelope / chemistry Nuclear Envelope / physiology Pachytene Stage / genetics Phenotype Point Mutation / genetics Synaptonemal Complex / genetics Synaptonemal Complex / metabolism X Chromosome / genetics X Chromosome / metabolism* Zinc Fingers / genetics|
|WOS Category||BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY|