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RRC ID 33971
Author Fujikawa Y, Morisaki F, Ogura A, Morohashi K, Enya S, Niwa R, Goto S, Kojima H, Okabe T, Nagano T, Inoue H.
Title A practical fluorogenic substrate for high-throughput screening of glutathione S-transferase inhibitors.
Journal Chem Commun (Camb)
Abstract We report a new fluorogenic substrate for glutathione S-transferase (GST), 3,4-DNADCF, enabling the assay with a low level of nonenzymatic background reaction. Inhibitors against Noppera-bo/GSTe14 from Drosophila melanogaster were identified by high throughput screening using 3,4-DNADCF, demonstrating the utility of this substrate.
Volume 51(57)
Pages 11459-62
Published 2015-7-21
DOI 10.1039/c5cc02067k
PMID 26090533
MeSH Animals Drosophila Proteins / antagonists & inhibitors* Drosophila Proteins / metabolism Drosophila melanogaster / enzymology* Drug Evaluation, Preclinical Enzyme Assays Enzyme Inhibitors / pharmacology* Fluorescent Dyes / chemistry Fluorescent Dyes / metabolism* Glutathione / chemistry Glutathione / metabolism Glutathione Transferase / antagonists & inhibitors* Glutathione Transferase / metabolism High-Throughput Screening Assays Humans Spectrometry, Fluorescence Substrate Specificity
IF 5.996
Times Cited 12
WOS Category CHEMISTRY, MULTIDISCIPLINARY
Resource
DNA material Genome Network human cDNA clone IRAL015K15 (HGY086255) IRAL037F09 (HGY094929) IRAK022O02 (HGX009138) IRAL007N16 (HGY083128) IRAL016K22 (HGY086662) IRAL005A23 (HGY082023) IRAK004O20 (HGX001956) IRAL006E09 (HGY082505) IRAL004F23 (HGY081743)