RRC ID 34090
Author Leong KH, Chen YS, Pan SF, Chen JJ, Wu HC, Chang YC, Yanagida F.
Title Diversity of lactic acid bacteria associated with fresh coffee cherries in Taiwan.
Journal Curr Microbiol
Abstract A total of 102 lactic acid bacteria (LAB) were isolated from three different coffee farms in Taiwan. These isolates were classified and identified by the restriction fragment length polymorphism analysis and sequencing of 16S ribosomal DNA. Heterofermentative Leuconostoc, and Weissella species were the most common LAB found in two farms located at an approximate altitude of 800 m. Lactococcus lactis subsp. lactis was the most common LAB found in the remaining farm was located at an approximate altitude of 1,200 m. It is therefore suggested that the altitude and climate may affect the distribution of LAB. On the basis of phylogenetic analysis, two strains included in the genera Enterococcus were considered as two potential novel species or subspecies. In addition, a total of 34 isolates showed the antifungal activity against Aspergillus flavus. Moreover, seven Lactococcus lactis subsp. lactis strains and one Enterococcus faecalis strain were found to have bacteriocin-like inhibitory substance-producing capability. These results suggest that various LAB are associated with fresh coffee cherries in Taiwan. Some of the isolates found in this study showed potential as antifungal agents.
Volume 68(4)
Pages 440-7
Published 2014-4-1
DOI 10.1007/s00284-013-0495-2
PMID 24292770
MeSH Coffee / microbiology* DNA, Bacterial / analysis DNA, Bacterial / genetics Fruit / microbiology* Lactobacillales / classification Lactobacillales / genetics Lactobacillales / isolation & purification* Phylogeny RNA, Ribosomal, 16S / analysis RNA, Ribosomal, 16S / genetics Taiwan
IF 1.746
Times Cited 19
General Microbes JCM 1157