論文 - 詳細
| RRC ID | 34156 |
|---|---|
| 著者 | Uchida M, Miyoshi T, Yoshida G, Niwa K, Mori M, Wakabayashi H. |
| タイトル | Isolation and characterization of halophilic lactic acid bacteria acting as a starter culture for sauce fermentation of the red alga Nori (Porphyra yezoensis). |
| ジャーナル | J Appl Microbiol |
| Abstract |
AIMS:A screening test was conducted for environmental samples to isolate halophilic lactic acid bacteria (HLAB) that can act as a starter in a Nori (Porphyra yezoensis)-sauce culture. METHOD AND RESULTS:After 9 months of incubation of enrichment cultures added with 25 kinds of environmental samples, growth of HLAB-like microorganisms was observed in six cultures salted at a 15% w/w level, including culture samples originally from mesopelagic water taken from 321 m-depth and from mountain snow taken at 2450 m-height. Ten strains were isolated and characterized as Tetragenococcus halophilus based on sequence analysis of the 16S rRNA gene. The isolates were inoculated into a newly prepared Nori-sauce culture and were confirmed to be able to act as a starter culture while three reference strains of T. halophilus obtained from a culture collection could not grow in the same culture. CONCLUSIONS:Halophilic lactic acid bacteria strains that can make growth in a highly salted Nori-sauce culture were isolated from environmental samples for the first time. All the isolates were identified as T. halophilus. SIGNIFICANCE AND IMPACT OF THE STUDY:The isolated strains are expected to be utilized as a starter culture for manufacturing fermented seaweed-sauce, which will be the first fermented food products obtained from algae. |
| 巻・号 | 116(6) |
| ページ | 1506-20 |
| 公開日 | 2014-6-1 |
| DOI | 10.1111/jam.12466 |
| PMID | 24494732 |
| MeSH | Colony Count, Microbial Enterococcaceae / classification* Enterococcaceae / isolation & purification Enterococcaceae / metabolism Fermentation* Food Microbiology* Lactic Acid Porphyra* RNA, Ribosomal, 16S / genetics Sodium Chloride |
| IF | 3.066 |
| 引用数 | 9 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| リソース情報 | |
| 一般微生物 | JCM 2015 JCM 5888 JCM 20245 |