RRC ID 342
Author Tanaka H, Takasu E, Aigaki T, Kato K, Hayashi S, Nose A.
Title Formin3 is required for assembly of the F-actin structure that mediates tracheal fusion in Drosophila.
Journal Dev. Biol.
Abstract During tracheal development in Drosophila, some branches join to form a continuous luminal network. Specialized cells at the branch tip, called fusion cells, extend filopodia to make contact and become doughnut shaped to allow passage of the lumen. These morphogenetic processes accompany the highly regulated cytoskeletal reorganization of fusion cells. We identified the Drosophila formin3 (form3) gene that encodes a novel formin and plays a role in tracheal fusion. Formins are a family of proteins characterized by highly conserved formin homology (FH) domains. The formin family functions in various actin-based processes, including cytokinesis and cell polarity. During embryogenesis, form3 mRNA is expressed mainly in the tracheal system. In form3 mutant embryos, the tracheal fusion does not occur at some points. This phenotype is rescued by the forced expression of form3 in the trachea. We used live imaging of GFP-moesin during tracheal fusion to show that an F-actin structure that spans the adjoining fusion cells and mediates the luminal connection does not form at abnormal anastomosis sites in form3 mutants. These results suggested that Form3 plays a role in the F-actin assembly, which is essential for cellular rearrangement during tracheal fusion.
Volume 274(2)
Pages 413-25
Published 2004-10-15
DOI 10.1016/j.ydbio.2004.07.035
PII S0012-1606(04)00508-1
PMID 15385168
MeSH Actins / chemistry Actins / metabolism* Amino Acid Sequence Animals Animals, Genetically Modified Axons / metabolism Cadherins / metabolism Cell Fusion Cytoskeleton / metabolism Drosophila Proteins / chemistry Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / anatomy & histology Drosophila melanogaster / embryology* Drosophila melanogaster / metabolism In Situ Hybridization Microfilament Proteins / chemistry Microfilament Proteins / genetics Microfilament Proteins / metabolism* Molecular Sequence Data Morphogenesis Phenotype Phylogeny Protein Structure, Tertiary Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism
IF 3.262
Times Cited 26
WOS Category DEVELOPMENTAL BIOLOGY
Resource
Drosophila