RRC ID 3429
Author Hayes GD, Frand AR, Ruvkun G.
Title The mir-84 and let-7 paralogous microRNA genes of Caenorhabditis elegans direct the cessation of molting via the conserved nuclear hormone receptors NHR-23 and NHR-25.
Journal Development
Abstract The let-7 microRNA (miRNA) gene of Caenorhabditis elegans controls the timing of developmental events. let-7 is conserved throughout bilaterian phylogeny and has multiple paralogs. Here, we show that the paralog mir-84 acts synergistically with let-7 to promote terminal differentiation of the hypodermis and the cessation of molting in C. elegans. Loss of mir-84 exacerbates phenotypes caused by mutations in let-7, whereas increased expression of mir-84 suppresses a let-7 null allele. Adults with reduced levels of mir-84 and let-7 express genes characteristic of larval molting as they initiate a supernumerary molt. mir-84 and let-7 promote exit from the molting cycle by regulating targets in the heterochronic pathway and also nhr-23 and nhr-25, genes encoding conserved nuclear hormone receptors essential for larval molting. The synergistic action of miRNA paralogs in development may be a general feature of the diversified miRNA gene family.
Volume 133(23)
Pages 4631-41
Published 2006-12
DOI 10.1242/dev.02655
PII dev.02655
PMID 17065234
MeSH 3' Untranslated Regions Animals Base Sequence Binding Sites / genetics Caenorhabditis elegans / genetics* Caenorhabditis elegans / growth & development* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Conserved Sequence DNA Primers / genetics DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Gene Expression Regulation, Developmental Genes, Helminth MicroRNAs / genetics* Models, Genetic Molting / genetics Molting / physiology Mutation RNA, Helminth / genetics* Receptors, Cytoplasmic and Nuclear / genetics Receptors, Cytoplasmic and Nuclear / metabolism* Sequence Homology, Nucleic Acid Transcription Factors / genetics Transcription Factors / metabolism*
IF 5.763
Times Cited 65
C.elegans tm1304