RRC ID |
3429
|
著者 |
Hayes GD, Frand AR, Ruvkun G.
|
タイトル |
The mir-84 and let-7 paralogous microRNA genes of Caenorhabditis elegans direct the cessation of molting via the conserved nuclear hormone receptors NHR-23 and NHR-25.
|
ジャーナル |
Development
|
Abstract |
The let-7 microRNA (miRNA) gene of Caenorhabditis elegans controls the timing of developmental events. let-7 is conserved throughout bilaterian phylogeny and has multiple paralogs. Here, we show that the paralog mir-84 acts synergistically with let-7 to promote terminal differentiation of the hypodermis and the cessation of molting in C. elegans. Loss of mir-84 exacerbates phenotypes caused by mutations in let-7, whereas increased expression of mir-84 suppresses a let-7 null allele. Adults with reduced levels of mir-84 and let-7 express genes characteristic of larval molting as they initiate a supernumerary molt. mir-84 and let-7 promote exit from the molting cycle by regulating targets in the heterochronic pathway and also nhr-23 and nhr-25, genes encoding conserved nuclear hormone receptors essential for larval molting. The synergistic action of miRNA paralogs in development may be a general feature of the diversified miRNA gene family.
|
巻・号 |
133(23)
|
ページ |
4631-41
|
公開日 |
2006-12-1
|
DOI |
10.1242/dev.02655
|
PII |
dev.02655
|
PMID |
17065234
|
MeSH |
3' Untranslated Regions
Animals
Base Sequence
Binding Sites / genetics
Caenorhabditis elegans / genetics*
Caenorhabditis elegans / growth & development*
Caenorhabditis elegans / metabolism
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Conserved Sequence
DNA Primers / genetics
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism*
Gene Expression Regulation, Developmental
Genes, Helminth
MicroRNAs / genetics*
Models, Genetic
Molting / genetics
Molting / physiology
Mutation
RNA, Helminth / genetics*
Receptors, Cytoplasmic and Nuclear / genetics
Receptors, Cytoplasmic and Nuclear / metabolism*
Sequence Homology, Nucleic Acid
Transcription Factors / genetics
Transcription Factors / metabolism*
|
IF |
5.611
|
引用数 |
75
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
|
リソース情報 |
線虫 |
tm1304 |