論文 - 詳細
| RRC ID | 3432 |
|---|---|
| 著者 | Hao L, Aspöck G, Bürglin TR. |
| タイトル | The hedgehog-related gene wrt-5 is essential for hypodermal development in Caenorhabditis elegans. |
| ジャーナル | Dev Biol |
| Abstract |
The Caenorhabditis elegans genome encodes a series of hedgehog-related genes, which are thought to have evolved and diverged from an ancestral Hh gene. They are classified into several families based on their N-terminal domains. Here, we analyze the expression and function of a member of the warthog gene family, wrt-5, that lacks the Hint/Hog domain. wrt-5 is expressed in seam cells, the pharynx, pharyngeal-intestinal valve cells, neurons, neuronal support cells, the excretory cell, and the reproductive system. WRT-5 protein is secreted into the extracellular space during embryogenesis. Furthermore, during larval development, WRT-5 protein is secreted into the pharyngeal lumen and the pharyngeal expression changes in a cyclical manner in phase with the molting cycle. Deletion mutations in wrt-5 cause embryonic lethality, which are temperature sensitive and more severe at 15 degrees C than at 25 degrees C. Animals that hatch exhibit variable abnormal morphology, for example, bagging worms, blistering, molting defects, or Roller phenotypes. We examined hypodermal cell junctions using the AJM-1Colon, two colonsGFP marker in the wrt-5 mutant background and observed cell boundary abnormalities in the arrested embryos. AJM-1Colon, two colonsGFP protein is also misplaced in pharyngeal muscle cells in the absence of WRT-5. In conclusion, we show that wrt-5 is an essential gene that - despite its lack of a Hint domain - has multiple functions in C. elegans and is implicated in cell shape integrity. |
| 巻・号 | 290(2) |
| ページ | 323-36 |
| 公開日 | 2006-2-15 |
| DOI | 10.1016/j.ydbio.2005.11.028 |
| PII | S0012-1606(05)00839-0 |
| PMID | 16413526 |
| MeSH | Alleles Amino Acid Sequence Animals Base Sequence Caenorhabditis Caenorhabditis elegans / embryology* Caenorhabditis elegans Proteins / biosynthesis Caenorhabditis elegans Proteins / physiology* Cell Communication Cloning, Molecular Cold Temperature Exons Gene Deletion Gene Expression Regulation, Developmental* Genetic Markers Genotype Green Fluorescent Proteins / metabolism Hedgehog Proteins Image Processing, Computer-Assisted Molecular Sequence Data Mutation Open Reading Frames Pharynx / embryology Phenotype Protein Structure, Tertiary Sequence Analysis, DNA Sequence Homology, Amino Acid Species Specificity Subcutaneous Tissue / embryology* Temperature Tissue Distribution Trans-Activators / biosynthesis Trans-Activators / metabolism Trans-Activators / physiology* |
| IF | 2.896 |
| 引用数 | 16 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
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| 線虫 | tm1084 |