RRC ID 3432
著者 Hao L, Aspöck G, Bürglin TR.
タイトル The hedgehog-related gene wrt-5 is essential for hypodermal development in Caenorhabditis elegans.
ジャーナル Dev Biol
Abstract The Caenorhabditis elegans genome encodes a series of hedgehog-related genes, which are thought to have evolved and diverged from an ancestral Hh gene. They are classified into several families based on their N-terminal domains. Here, we analyze the expression and function of a member of the warthog gene family, wrt-5, that lacks the Hint/Hog domain. wrt-5 is expressed in seam cells, the pharynx, pharyngeal-intestinal valve cells, neurons, neuronal support cells, the excretory cell, and the reproductive system. WRT-5 protein is secreted into the extracellular space during embryogenesis. Furthermore, during larval development, WRT-5 protein is secreted into the pharyngeal lumen and the pharyngeal expression changes in a cyclical manner in phase with the molting cycle. Deletion mutations in wrt-5 cause embryonic lethality, which are temperature sensitive and more severe at 15 degrees C than at 25 degrees C. Animals that hatch exhibit variable abnormal morphology, for example, bagging worms, blistering, molting defects, or Roller phenotypes. We examined hypodermal cell junctions using the AJM-1Colon, two colonsGFP marker in the wrt-5 mutant background and observed cell boundary abnormalities in the arrested embryos. AJM-1Colon, two colonsGFP protein is also misplaced in pharyngeal muscle cells in the absence of WRT-5. In conclusion, we show that wrt-5 is an essential gene that - despite its lack of a Hint domain - has multiple functions in C. elegans and is implicated in cell shape integrity.
巻・号 290(2)
ページ 323-36
公開日 2006-2-15
DOI 10.1016/j.ydbio.2005.11.028
PII S0012-1606(05)00839-0
PMID 16413526
MeSH Alleles Amino Acid Sequence Animals Base Sequence Caenorhabditis Caenorhabditis elegans / embryology* Caenorhabditis elegans Proteins / biosynthesis Caenorhabditis elegans Proteins / physiology* Cell Communication Cloning, Molecular Cold Temperature Exons Gene Deletion Gene Expression Regulation, Developmental* Genetic Markers Genotype Green Fluorescent Proteins / metabolism Hedgehog Proteins Image Processing, Computer-Assisted Molecular Sequence Data Mutation Open Reading Frames Pharynx / embryology Phenotype Protein Structure, Tertiary Sequence Analysis, DNA Sequence Homology, Amino Acid Species Specificity Subcutaneous Tissue / embryology* Temperature Tissue Distribution Trans-Activators / biosynthesis Trans-Activators / metabolism Trans-Activators / physiology*
IF 2.896
引用数 16
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
線虫 tm1084