RRC ID 34528
Author Levi-Ferber M, Salzberg Y, Safra M, Haviv-Chesner A, Bülow HE, Henis-Korenblit S.
Title It's all in your mind: determining germ cell fate by neuronal IRE-1 in C. elegans.
Journal PLoS Genet
Abstract The C. elegans germline is pluripotent and mitotic, similar to self-renewing mammalian tissues. Apoptosis is triggered as part of the normal oogenesis program, and is increased in response to various stresses. Here, we examined the effect of endoplasmic reticulum (ER) stress on apoptosis in the C. elegans germline. We demonstrate that pharmacological or genetic induction of ER stress enhances germline apoptosis. This process is mediated by the ER stress response sensor IRE-1, but is independent of its canonical downstream target XBP-1. We further demonstrate that ire-1-dependent apoptosis in the germline requires both CEP-1/p53 and the same canonical apoptotic genes as DNA damage-induced germline apoptosis. Strikingly, we find that activation of ire-1, specifically in the ASI neurons, but not in germ cells, is sufficient to induce apoptosis in the germline. This implies that ER stress related germline apoptosis can be determined at the organism level, and is a result of active IRE-1 signaling in neurons. Altogether, our findings uncover ire-1 as a novel cell non-autonomous regulator of germ cell apoptosis, linking ER homeostasis in sensory neurons and germ cell fate.
Volume 10(10)
Pages e1004747
Published 2014-10-1
DOI 10.1371/journal.pgen.1004747
PMID 25340700
PMC PMC4207656
MeSH Animals Apoptosis / genetics Caenorhabditis elegans Caenorhabditis elegans Proteins / genetics* Carrier Proteins / genetics Cell Differentiation / genetics* DNA Damage / genetics Endoplasmic Reticulum Stress / genetics Germ Cells / growth & development Gonads / growth & development Neurons / cytology* Protein Serine-Threonine Kinases / genetics* Signal Transduction*
IF 5.175
Times Cited 14
C.elegans tm2457 tm2308 tm2482 tm536