RRC ID 34554
Author Takaine M, Numata O, Nakano K.
Title An actin-myosin-II interaction is involved in maintaining the contractile ring in fission yeast.
Journal J Cell Sci
Abstract The actomyosin-based contractile ring, which assembles at the cell equator, maintains its circularity during cytokinesis in many eukaryotic cells, ensuring its efficient constriction. Although consistent maintenance of the ring is one of the mechanisms underpinning cytokinesis, it has not yet been fully addressed. We here investigated the roles of fission yeast myosin-II proteins [Myo2 and Myo3 (also known as Myp2)] in ring maintenance during cytokinesis, with a focus on Myo3. A site-directed mutational analysis showed that the motor properties of Myo3 were involved in its accumulation in the contractile ring. The assembled ring was often deformed and not properly maintained under conditions in which the activities of myosin-II proteins localizing to the contractile ring were decreased, leading to inefficient cell division. Moreover, Myo3 appeared to form motile clusters on the ring. We propose that large assemblies of myosin-II proteins consolidate the contractile ring by continuously binding to F-actin in the ring, thereby contributing to its maintenance.
Volume 128(15)
Pages 2903-18
Published 2015-8-1
DOI 10.1242/jcs.171264
PII jcs.171264
PMID 26092938
MeSH Actin Cytoskeleton / metabolism* Actinin / genetics Actins / metabolism Cell Cycle Proteins / genetics Cell Division / physiology Cytokinesis / physiology* Myosin Heavy Chains / genetics Myosin Heavy Chains / metabolism* Myosin Type II / genetics Myosin Type II / metabolism* Schizosaccharomyces / genetics* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism*
IF 4.573
Times Cited 11
Yeast FY12017 FY13589 FY13592 FY14012