RRC ID 346
Author Chiang AS, Blum A, Barditch J, Chen YH, Chiu SL, Regulski M, Armstrong JD, Tully T, Dubnau J.
Title radish encodes a phospholipase-A2 and defines a neural circuit involved in anesthesia-resistant memory.
Journal Curr. Biol.
Abstract BACKGROUND:In both vertebrate and invertebrate animals, anesthetic agents cause retrograde amnesia for recently experienced events. In contrast, older memories are resistant to the same treatments. In Drosophila, anesthesia-resistant memory (ARM) and long-term memory (LTM) are genetically distinct forms of long-lasting memory that exist in parallel for at least a day after training. ARM is disrupted in radish mutants but is normal in transgenic flies overexpressing a CREB repressor transgene. In contrast, LTM is normal in radish mutants but is disrupted in CREB repressor transgenic flies. To date, nothing is known about the molecular, genetic, or cell biological pathways underlying ARM.
RESULTS:Here, we report the molecular identification of radish as a phospholipase-A2, providing the first clue about signaling pathways underlying ARM in any animal. An enhancer-trap allele of radish (C133) reveals expression in a novel anatomical pathway. Transgenic expression of PLA2 under control of C133 restores normal levels of ARM to radish mutants, whereas transient disruption of neural activity in C133 neurons inhibits memory retention. Notably, expression of C133 is not in mushroom bodies, the primary anatomical focus of olfactory memory research in Drosophila.
CONCLUSIONS:Identification of radish as a phospholipase-A2 and the neural expression pattern of an enhancer-trap allele significantly broaden our understanding of the biochemistry and anatomy underlying olfactory memory in Drosophila.
Volume 14(4)
Pages 263-72
Published 2004-2-17
DOI 10.1016/j.cub.2004.02.003
PII S0960-9822(04)00083-1
PMID 14972677
MeSH Alleles Amino Acid Sequence Anesthesia* Animals Animals, Genetically Modified Blotting, Northern Blotting, Western Brain / metabolism Cold Temperature* Crosses, Genetic DNA Primers Drosophila melanogaster / genetics Drosophila melanogaster / physiology* Enhancer Elements, Genetic / genetics Gene Expression Profiling Genes, Reporter / genetics Green Fluorescent Proteins Luminescent Proteins / metabolism Memory / physiology* Microscopy, Fluorescence Molecular Sequence Data Phospholipases A / genetics* Phospholipases A2 Plasmids / genetics Signal Transduction / physiology Smell / physiology
IF 9.251
Times Cited 22
WOS Category CELL BIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Drosophila