RRC ID 34784
Author Takeda H, Inoue H, Kutsuna T, Matsushita N, Takahashi T, Watanabe S, Higashiyama S, Yamamoto H.
Title Activation of epidermal growth factor receptor gene is involved in transforming growth factor-beta-mediated fibronectin expression in a chondrocyte progenitor cell line.
Journal Int J Mol Med
Abstract Chondrocytes produce many types of ECM to maintain elasticity and plasticity in articular cartilage of revolute joints. Both transforming growth factor beta (TGF-beta) and bone morphogenetic proteins (BMPs) induce extracellular matrix proteins such as type IIalpha1 collagen and aggrecan during chondrogenic differentiation in vitro. However, differences in the matrix gene expression pattern by the stimulation of TGF-betas and BMPs remains unclear. In the present study, we created a customized PCR-based ECM array to investigate the pattern of ECM expression genes in the chondrocyte progenitor cell line ATDC5, that was stimulated by TGF-betas or BMPs. Fibronectin (Fn) expression was drastically induced after TGF-beta stimulation, but not BMP-4. Epidermal growth factor receptor (Egfr) gene was also significantly activated in TGF-beta1-induced chondrogenic differentiation as compared to BMP-4-mediated differentiation. Furthermore, EGFR-knockdown assay of the cells showed decreasing Fn expression during TGF-beta1-induced chondrogenic differentiation. These data indicated that Egfr gene activation by TGF-beta is involved in the differences in the expression of cellular matrix genes such as Fn, as compared to the expression pattern induced by BMPs.
Volume 25(4)
Pages 593-600
Published 2010-4-1
DOI 10.3892/ijmm_00000381
PMID 20198308
MeSH Animals Bone Morphogenetic Protein 4 / pharmacology Cell Differentiation / drug effects Cell Differentiation / genetics Cell Line Chondrocytes / cytology* Chondrocytes / drug effects Chondrocytes / metabolism* Down-Regulation / drug effects ErbB Receptors / genetics* ErbB Receptors / metabolism Extracellular Matrix / drug effects Extracellular Matrix / genetics Extracellular Matrix / metabolism Fibronectins / genetics* Fibronectins / metabolism Gene Expression Profiling Gene Knockdown Techniques Humans Mice RNA, Small Interfering / metabolism Stem Cells / drug effects Stem Cells / metabolism* Transcriptional Activation* / drug effects Transforming Growth Factor beta / pharmacology* Up-Regulation / drug effects
IF 3.098
Times Cited 14
DNA material CSII-CMV-MCS-IRES2-Bsd (RDB04385) pCAG-HIVgp (RDB04394) pCMV-VSV-G-RSV-Rev (RDB04393)
Human and Animal Cells ATDC5(RCB0565)