RRC ID 34806
Author Narita Y, Sugimoto A, Kawashima D, Watanabe T, Kanda T, Kimura H, Tsurumi T, Murata T.
Title A Herpesvirus Specific Motif of Epstein-Barr Virus DNA Polymerase Is Required for the Efficient Lytic Genome Synthesis.
Journal Sci Rep
Abstract Epstein-Barr virus (EBV) is associated with several malignancies, including Burkitt lymphoma and nasopharyngeal carcinoma. To overcome such disorders, understanding the molecular mechanisms of the EBV replication is important. The EBV DNA polymerase (Pol) is one of the essential factors for viral lytic DNA replication. Although it is well known that its C-terminal half, possessing DNA polymerase and 3'-5' exonuclease activity, is highly conserved among Family B Pols, the NH2-terminal half has yet to be characterized in detail. In this study, we show that a stretch of hydrophobic amino acids within the pre-NH2-terminal domain of EBV Pol plays important role. In addition, we could identify the most essential residue for replication in the motif. These findings will shed light on molecular mechanisms of viral DNA synthesis and will help to develop new herpesviruses treatments.
Volume 5
Pages 11767
Published 2015-6-30
DOI 10.1038/srep11767
PII srep11767
PMID 26123572
PMC PMC4485236
MeSH Amino Acid Sequence Conserved Sequence DNA Replication DNA, Viral / genetics DNA, Viral / metabolism DNA-Directed DNA Polymerase / chemistry* DNA-Directed DNA Polymerase / metabolism Genome, Viral HEK293 Cells Herpesvirus 4, Human / enzymology* Herpesvirus 4, Human / genetics Humans Hydrophobic and Hydrophilic Interactions Molecular Sequence Data Viral Proteins / chemistry* Viral Proteins / metabolism Virus Replication*
IF 3.998
Times Cited 4
DNA material pcDNA3.1(-) BALF5 (RDB13542) pcDNA3.1(-) BALF5 hsm 6A (RDB13543) pcDNA3.1(-) FLAG-BALF5 (RDB13544) pcDNA3.1(-) FLAG-BALF5 hsm 6A (RDB13545) pcDNA3.1(-) HA-BSLF1 (RDB13546) pcDNA3.1(-) HA-BSLF2/3 (RDB13547) pcDNA3.1(-) HA-BSLF4 (RDB13548).