RRC ID 34829
Author Naka H, Nakamura S, Shimazaki T, Okano H.
Title Requirement for COUP-TFI and II in the temporal specification of neural stem cells in CNS development.
Journal Nat Neurosci
Abstract In the developing CNS, subtypes of neurons and glial cells are generated according to a schedule that is defined by cell-intrinsic mechanisms that function at the progenitor-cell level. However, no critical molecular switch for the temporal specification of CNS progenitor cells has been identified. We found that chicken ovalbumin upstream promoter-transcription factor I and II (Coup-tfI and Coup-tfII, also known as Nr2f1 and Nr2f2) are required for the temporal specification of neural stem/progenitor cells (NSPCs), including their acquisition of gliogenic competence, as demonstrated by their responsiveness to gliogenic cytokines. COUP-TFI and II are transiently co-expressed in the ventricular zone of the early embryonic CNS. The double knockdown of Coup-tfI/II in embryonic stem cell (ESC)-derived NSPCs and the developing mouse forebrain caused sustained neurogenesis and the prolonged generation of early-born neurons. These findings reveal a part of the timer mechanisms for generating diverse types of neurons and glial cells during CNS development.
Volume 11(9)
Pages 1014-23
Published 2008-9-1
DOI 10.1038/nn.2168
PII nn.2168
PMID 19160499
MeSH Animals Bromodeoxyuridine / metabolism COUP Transcription Factor I / genetics COUP Transcription Factor I / metabolism* COUP Transcription Factor II / genetics COUP Transcription Factor II / metabolism* Cell Differentiation / drug effects Cell Differentiation / physiology* Cells, Cultured Central Nervous System / cytology* Central Nervous System / drug effects Central Nervous System / embryology* Chromatin Immunoprecipitation / methods Cytokines / metabolism Embryo, Mammalian Gene Expression Regulation, Developmental Glial Fibrillary Acidic Protein / metabolism Green Fluorescent Proteins / genetics Mice Mice, Inbred ICR Mutation / genetics Neurogenesis / genetics Neuroglia / physiology Neurons / drug effects Neurons / physiology* Organ Culture Techniques RNA, Small Interfering / pharmacology Stem Cells / drug effects Stem Cells / physiology* Time Factors Transfection / methods Tubulin / metabolism
IF 20.071
Times Cited 145
DNA material pCAG-HIVgp (RDB04394) pCMV-VSV-G-RSV-Rev (RDB04393) CSII-EF-MCS-IRES2-Venus (RDB04384) CSII-EF-RfA-IRES2-Venus (RDB04389) CS-RfA-EG (RDB04391).