Reference - Detail
|Author||Naka H, Nakamura S, Shimazaki T, Okano H.|
|Title||Requirement for COUP-TFI and II in the temporal specification of neural stem cells in CNS development.|
In the developing CNS, subtypes of neurons and glial cells are generated according to a schedule that is defined by cell-intrinsic mechanisms that function at the progenitor-cell level. However, no critical molecular switch for the temporal specification of CNS progenitor cells has been identified. We found that chicken ovalbumin upstream promoter-transcription factor I and II (Coup-tfI and Coup-tfII, also known as Nr2f1 and Nr2f2) are required for the temporal specification of neural stem/progenitor cells (NSPCs), including their acquisition of gliogenic competence, as demonstrated by their responsiveness to gliogenic cytokines. COUP-TFI and II are transiently co-expressed in the ventricular zone of the early embryonic CNS. The double knockdown of Coup-tfI/II in embryonic stem cell (ESC)-derived NSPCs and the developing mouse forebrain caused sustained neurogenesis and the prolonged generation of early-born neurons. These findings reveal a part of the timer mechanisms for generating diverse types of neurons and glial cells during CNS development.
|MeSH||Animals Bromodeoxyuridine / metabolism COUP Transcription Factor I / genetics COUP Transcription Factor I / metabolism* COUP Transcription Factor II / genetics COUP Transcription Factor II / metabolism* Cell Differentiation / drug effects Cell Differentiation / physiology* Cells, Cultured Central Nervous System / cytology* Central Nervous System / drug effects Central Nervous System / embryology* Chromatin Immunoprecipitation / methods Cytokines / metabolism Embryo, Mammalian Gene Expression Regulation, Developmental Glial Fibrillary Acidic Protein / metabolism Green Fluorescent Proteins / genetics Mice Mice, Inbred ICR Mutation / genetics Neurogenesis / genetics Neuroglia / physiology Neurons / drug effects Neurons / physiology* Organ Culture Techniques RNA, Small Interfering / pharmacology Stem Cells / drug effects Stem Cells / physiology* Time Factors Transfection / methods Tubulin / metabolism|
|DNA material||pCAG-HIVgp (RDB04394) pCMV-VSV-G-RSV-Rev (RDB04393) CSII-EF-MCS-IRES2-Venus (RDB04384) CSII-EF-RfA-IRES2-Venus (RDB04389) CS-RfA-EG (RDB04391).|