論文 - 詳細
| RRC ID | 34876 |
|---|---|
| 著者 | Pradhan-Sundd T, Verheyen EM. |
| タイトル | The Myopic-Ubpy-Hrs nexus enables endosomal recycling of Frizzled. |
| ジャーナル | Mol Biol Cell |
| Abstract |
Endosomal trafficking of signaling proteins plays an essential role in cellular homeostasis. The seven-pass transmembrane protein Frizzled (Fz) is a critical component of Wnt signaling. Although Wnt signaling is proposed to be regulated by endosomal trafficking of Fz, the molecular events that enable this regulation are not completely understood. Here we show that the endosomal protein Myopic (Mop) regulates Fz trafficking in the Drosophila wing disk by inhibiting the ubiquitination and degradation of Hrs. Deletion of Mop or Hrs results in endosomal accumulation of Fz and therefore reduced Wnt signaling. The in situ proximity ligation assay revealed a strong association between Mop and Hrs in the Drosophila wing disk. Overexpression of Hrs rescues the trafficking defect caused by mop knockdown. Mop aids in the maintenance of Ubpy, which deubiquitinates (and thus stabilizes) Hrs. In the absence of the ubiquitin ligase Cbl, Mop is dispensable. These findings support a previously unknown role for Mop in endosomal trafficking of Fz in Wnt-receiving cells. |
| 巻・号 | 26(18) |
| ページ | 3329-42 |
| 公開日 | 2015-9-15 |
| DOI | 10.1091/mbc.E15-02-0086 |
| PII | mbc.E15-02-0086 |
| PMID | 26224310 |
| PMC | PMC4569321 |
| MeSH | Animals Drosophila Proteins / metabolism* Drosophila melanogaster Endocytosis Endopeptidases / metabolism Endosomal Sorting Complexes Required for Transport / metabolism* Endosomes / metabolism Frizzled Receptors / metabolism* Phosphoproteins / metabolism* Protein Transport Protein Tyrosine Phosphatases / metabolism* Signal Transduction Ubiquitin / metabolism Wnt Signaling Pathway |
| IF | 3.791 |
| 引用数 | 8 |
| WOS 分野 | CELL BIOLOGY |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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