Reference - Detail
| RRC ID | 34899 |
|---|---|
| Author | Murata T, Ishitsuka Y, Karouji K, Kaneda H, Toki H, Nakai Y, Makino S, Fukumura R, Kotaki H, Wakana S, Noda T, Gondo Y. |
| Title | β-CateninC429S mice exhibit sterility consequent to spatiotemporally sustained Wnt signalling in the internal genitalia. |
| Journal | Sci Rep |
| Abstract |
Wnt/β-catenin signalling regulates numerous developmental and homeostatic processes. Ctnnb1 (also known as β-catenin) is the only protein that transmits signals from various Wnt ligands to downstream genes. In this study, we report that our newly established mouse strain, which harbours a Cys429 to Ser missense mutation in the β-catenin gene, exhibited specific organ defects in contrast to mice with broadly functioning Wnt/β-catenin signalling. Both homozygous mutant males and females produced normal gametes but were infertile because of abnormal seminal vesicle and vaginal morphogenesis. An ins-TOPGAL transgenic reporter spatiotemporally sustained Wnt/β-catenin signalling during the corresponding organogenesis. Therefore, β-catenin(C429S) should provide new insights into β-catenin as a universal component of Wnt/β-catenin signal transduction. |
| Volume | 4 |
| Pages | 6959 |
| Published | 2014-11-7 |
| DOI | 10.1038/srep06959 |
| PII | srep06959 |
| PMID | 25376241 |
| PMC | PMC4223658 |
| MeSH | Animals Embryo, Mammalian Female Genes, Reporter Homozygote Infertility, Female / genetics* Infertility, Male / genetics* Male Mice Mice, Inbred C57BL Mice, Transgenic Mutation* Ovum / growth & development Ovum / metabolism Seminal Vesicles / abnormalities Seminal Vesicles / growth & development Seminal Vesicles / metabolism* Spermatozoa / growth & development Spermatozoa / metabolism Vagina / abnormalities Vagina / growth & development Vagina / metabolism* Wnt Proteins / genetics Wnt Proteins / metabolism Wnt Signaling Pathway* beta Catenin / genetics* beta Catenin / metabolism |
| IF | 3.998 |
| Times Cited | 3 |
| WOS Category | DEVELOPMENTAL BIOLOGY |
| Resource | |
| DNA material | CMV-V5-CTNNB1(WT) (RDB13062) CMV-V5-CTNNB1(T42I) (RDB13063) CMV-V5-CTNNB1(V195E) (RDB13064) CMV-V5-CTNNB1(K233E) (RDB13065) CMV-V5-CTNNB1(C419*) (RDB13066) CMV-V5-CTNNB1(C429S) (RDB13067) CMV-V5-CTNNB1(H578Q) (RDB13068) CMV-V5-CTNNB1(V584I) (RDB13069) CMV-V5-CTNNB1(S45A) (RDB13070) CMV-GFP-CTNNB1(WT) (RDB13071) CMV-GFP-CTNNB1(T42I) (RDB13072) CMV-GFP-CTNNB1(V195E) (RDB13073) CMV-GFP-CTNNB1(K233E) (RDB13074) CMV-GFP-CTNNB1(C419*) (RDB13075) CMV-GFP-CTNNB1(C429S) (RDB13076) CMV-GFP-CTNNB1(H578Q) (RDB13077) CMV-GFP-CTNNB1(V584I) (RDB13078) CMV-GFP-CTNNB1(S45A) (RDB13079) |