論文 - 詳細
| RRC ID | 34905 |
|---|---|
| 著者 | Yang HJ, Asakawa H, Haraguchi T, Hiraoka Y. |
| タイトル | Nup132 modulates meiotic spindle attachment in fission yeast by regulating kinetochore assembly. |
| ジャーナル | J Cell Biol |
| Abstract |
During meiosis, the kinetochore undergoes substantial reorganization to establish monopolar spindle attachment. In the fission yeast Schizosaccharomyces pombe, the KNL1-Spc7-Mis12-Nuf2 (KMN) complex, which constitutes the outer kinetochore, is disassembled during meiotic prophase and is reassembled before meiosis I. Here, we show that the nucleoporin Nup132 is required for timely assembly of the KMN proteins: In the absence of Nup132, Mis12 and Spc7 are precociously assembled at the centromeres during meiotic prophase. In contrast, Nuf2 shows timely dissociation and reappearance at the meiotic centromeres. We further demonstrate that depletion of Nup132 activates the spindle assembly checkpoint in meiosis I, possibly because of the increased incidence of erroneous spindle attachment at sister chromatids. These results suggest that precocious assembly of the kinetochores leads to the meiosis I defects observed in the nup132-disrupted mutant. Thus, we propose that Nup132 plays an important role in establishing monopolar spindle attachment at meiosis I through outer kinetochore reorganization at meiotic prophase. |
| 巻・号 | 211(2) |
| ページ | 295-308 |
| 公開日 | 2015-10-26 |
| DOI | 10.1083/jcb.201501035 |
| PII | jcb.201501035 |
| PMID | 26483559 |
| PMC | PMC4621824 |
| MeSH | Cell Cycle Proteins / metabolism Chromatids / genetics Chromosomal Proteins, Non-Histone / metabolism Chromosome Segregation / genetics Gene Deletion Kinetochores / metabolism* Meiosis / genetics Microscopy, Fluorescence Microtubule-Associated Proteins / metabolism Multiprotein Complexes / metabolism Nuclear Pore Complex Proteins / genetics Nuclear Pore Complex Proteins / metabolism* Nuclear Proteins / metabolism Polymerase Chain Reaction Protein Serine-Threonine Kinases / metabolism Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Spindle Apparatus / metabolism* |
| IF | 8.811 |
| 引用数 | 6 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | FY13800 |