論文 - 詳細
| RRC ID | 34920 |
|---|---|
| 著者 | Maguire JE, Silva M, Nguyen KC, Hellen E, Kern AD, Hall DH, Barr MM. |
| タイトル | Myristoylated CIL-7 regulates ciliary extracellular vesicle biogenesis. |
| ジャーナル | Mol Biol Cell |
| Abstract |
The cilium both releases and binds to extracellular vesicles (EVs). EVs may be used by cells as a form of intercellular communication and mediate a broad range of physiological and pathological processes. The mammalian polycystins (PCs) localize to cilia, as well as to urinary EVs released from renal epithelial cells. PC ciliary trafficking defects may be an underlying cause of autosomal dominant polycystic kidney disease (PKD), and ciliary-EV interactions have been proposed to play a central role in the biology of PKD. In Caenorhabditis elegans and mammals, PC1 and PC2 act in the same genetic pathway, act in a sensory capacity, localize to cilia, and are contained in secreted EVs, suggesting ancient conservation. However, the relationship between cilia and EVs and the mechanisms generating PC-containing EVs remain an enigma. In a forward genetic screen for regulators of C. elegans PKD-2 ciliary localization, we identified CIL-7, a myristoylated protein that regulates EV biogenesis. Loss of CIL-7 results in male mating behavioral defects, excessive accumulation of EVs in the lumen of the cephalic sensory organ, and failure to release PKD-2::GFP-containing EVs to the environment. Fatty acylation, such as myristoylation and palmitoylation, targets proteins to cilia and flagella. The CIL-7 myristoylation motif is essential for CIL-7 function and for targeting CIL-7 to EVs. C. elegans is a powerful model with which to study ciliary EV biogenesis in vivo and identify cis-targeting motifs such as myristoylation that are necessary for EV-cargo association and function. |
| 巻・号 | 26(15) |
| ページ | 2823-32 |
| 公開日 | 2015-8-1 |
| DOI | 10.1091/mbc.E15-01-0009 |
| PII | mbc.E15-01-0009 |
| PMID | 26041936 |
| PMC | PMC4571341 |
| MeSH | Acylation Animals Biological Transport Caenorhabditis elegans Caenorhabditis elegans Proteins / metabolism Cilia / metabolism* Extracellular Vesicles / metabolism* Humans Male Microscopy, Electron, Transmission Models, Animal Myristates / metabolism Polycystic Kidney Diseases / metabolism TRPP Cation Channels / metabolism |
| IF | 3.791 |
| 引用数 | 22 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm5848 tm925 |