Reference - Detail
|Author||Ishiguro K, Kim J, Fujiyama-Nakamura S, Kato S, Watanabe Y.|
|Title||A new meiosis-specific cohesin complex implicated in the cohesin code for homologous pairing.|
We identify a new mammalian cohesin subunit, RAD21-like protein (RAD21L), with sequence similarity to RAD21 and REC8. RAD21L localizes along axial elements in early meiotic prophase, in a manner that is spatiotemporally different to either REC8 or RAD21. Remarkably, RAD21L and REC8 have symmetrical, mutually exclusive localization on the not-yet-synapsed homologues, implying that the cohesin patterning could provide a code for homologue recognition. RAD21 transiently localizes to axial elements after the dissociation of RAD21L and REC8 in late pachytene, a period of recombination repair. Further, we show that the removal of cohesins and synaptonemal complex during late meiotic prophase is promoted by Polo-like kinase 1, which is similar to the mitotic prophase pathway.
|MeSH||Amino Acid Sequence Animals Cell Cycle Proteins / chemistry Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Centromere Chromatography, Liquid Chromosomal Proteins, Non-Histone / chemistry Chromosomal Proteins, Non-Histone / genetics Chromosomal Proteins, Non-Histone / metabolism* Chromosome Pairing* Chromosome Segregation Chromosomes, Mammalian / metabolism* Mass Spectrometry Meiosis* Mice Molecular Sequence Data Nuclear Proteins / metabolism Phosphoproteins / metabolism Prophase Protein Subunits Protein-Serine-Threonine Kinases / metabolism Proto-Oncogene Proteins / metabolism Synaptonemal Complex / metabolism|
|WOS Category||BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY|
|DNA material||pCRII-mRAD21L (RDB12167)|