RRC ID 34928
Author Rossio V, Michimoto T, Sasaki T, Ohbayashi I, Kikuchi Y, Yoshida S.
Title Nuclear PP2A-Cdc55 prevents APC-Cdc20 activation during the spindle assembly checkpoint.
Journal J Cell Sci
Abstract Cdc55, a regulatory B-subunit of protein phosphatase 2A (PP2A) complex, is essential for the spindle assembly checkpoint (SAC) in budding yeast, but the regulation and molecular targets of PP2A-Cdc55 have not been clearly defined or are controversial. Here, we show that an important target of Cdc55 in the SAC is the anaphase-promoting complex (APC) coupled with Cdc20 and that APC-Cdc20 is kept inactive by dephosphorylation by nuclear PP2A-Cdc55 when spindle is damaged. By isolating a new class of Cdc55 mutants specifically defective in the SAC and by artificially manipulating nucleocytoplasmic distribution of Cdc55, we further show that nuclear Cdc55 is essential for the SAC. Because the Cdc55-binding proteins Zds1 and Zds2 inhibit both nuclear accumulation of Cdc55 and SAC activity, we propose that spatial control of PP2A by Zds1 family proteins is important for tight control of SAC and mitotic progression.
Volume 126(Pt 19)
Pages 4396-405
Published 2013-10-1
DOI 10.1242/jcs.127365
PII jcs.127365
PMID 23886942
MeSH Alleles Amino Acid Sequence Anaphase-Promoting Complex-Cyclosome / genetics Anaphase-Promoting Complex-Cyclosome / metabolism* Cdc20 Proteins / metabolism* Cell Cycle Proteins / chemistry Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Humans Models, Molecular Molecular Sequence Data Nuclear Proteins / metabolism Phosphorylation Protein Phosphatase 2 / chemistry Protein Phosphatase 2 / genetics Protein Phosphatase 2 / metabolism* Saccharomyces cerevisiae / enzymology Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism* Saccharomyces cerevisiae Proteins / chemistry Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism* Spindle Apparatus / enzymology Spindle Apparatus / metabolism*
IF 4.573
Times Cited 7
Yeast BYP1613(ZDS1)