RRC ID 35143
著者 Shahsavarani H, Sugiyama M, Kaneko Y, Chuenchit B, Harashima S.
タイトル Superior thermotolerance of Saccharomyces cerevisiae for efficient bioethanol fermentation can be achieved by overexpression of RSP5 ubiquitin ligase.
ジャーナル Biotechnol Adv
Abstract The simultaneous saccharification and fermentation process requires thermo-tolerant yeast to facilitate the enzymatic hydrolysis of cellulose. In this paper, we describe a Htg+ strain that exhibits confluent growth at high temperature (41 °C) and resistance to heat shock, ethanol, osmotic, oxidative and DNA damage stresses. HTG6, one of the six genes responsible for the thermotolerant phenotype was identified to be the gene RSP5 encoding a ubiquitin ligase. The RSP5 allele of the Htg+ strain, designated RSP5-C, possessed five, one and two base changes in the promoter, open reading frame and terminator region, respectively. The base changes in the promoter region of the RSP5-C allele were found to be responsible for the thermotolerant phenotype by strongly increasing transcription of the RSP5 gene and consequently causing a rise in the ubiquitination of cell proteins. Overexpression of the RSP5-BY allele present in the htg6 host strain (Htg-) conferred thermotolerance at 41°C, to this strain as in the case of RSP5-C allele. We also discovered that an Htg+ strain overexpressing the RSP5-C allele exhibits a more robust Htg+ phenotype against higher temperature (43 °C). The data presented here also suggest that overexpression of RSP5 could be applied to raise the upper limit of thermotolerance in S. cerevisiae strain used for industrial bioethanol production.
巻・号 30(6)
ページ 1289-300
公開日 2012-1-1
DOI 10.1016/j.biotechadv.2011.09.002
PII S0734-9750(11)00155-8
PMID 21930195
MeSH Adaptation, Physiological* / drug effects Alleles Base Sequence Biofuels / microbiology* Cell Wall / drug effects Cell Wall / metabolism Chromosome Mapping Cytoprotection / drug effects DNA Damage Endosomal Sorting Complexes Required for Transport / genetics* Endosomal Sorting Complexes Required for Transport / metabolism Ethanol / metabolism* Ethanol / toxicity Fermentation / drug effects Fermentation / physiology* Genes, Fungal / genetics Heat-Shock Response / drug effects Microbial Viability / drug effects Microbial Viability / genetics Mutation / genetics Osmotic Pressure / drug effects Oxidative Stress / drug effects Phenotype Promoter Regions, Genetic / genetics Saccharomyces cerevisiae / cytology Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / growth & development Saccharomyces cerevisiae / physiology* Saccharomyces cerevisiae Proteins / genetics* Saccharomyces cerevisiae Proteins / metabolism Temperature* Transcription, Genetic / drug effects Ubiquitin-Protein Ligase Complexes / genetics* Ubiquitin-Protein Ligase Complexes / metabolism Ubiquitination / drug effects Ubiquitination / genetics
IF 10.744
引用数 42
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
リソース情報
酵母 BYP5166 BYP555 BYP353