論文 - 詳細
| RRC ID | 35146 |
|---|---|
| 著者 | Sugiyama M, Nugroho S, Iida N, Sakai T, Kaneko Y, Harashima S. |
| タイトル | Genetic interactions of ribosome maturation factors Yvh1 and Mrt4 influence mRNA decay, glycogen accumulation, and the expression of early meiotic genes in Saccharomyces cerevisiae. |
| ジャーナル | J Biochem |
| Abstract |
The Saccharomyces cerevisiae Yvh1, a dual-specificity protein phosphatase involved in glycogen accumulation and sporulation, is required for normal vegetative growth. To further elucidate the role of Yvh1, we generated dominant mutants suppressing the slow growth caused by YVH1 disruption. One of the mutant alleles, designated as SVH1-1 (suppressor of Δyvh1 deletion), was identical to MRT4 (mRNA turnover) that contained a single-base substitution causing an amino acid change from Gly(68) to Asp. Mrt4(G68D) restored the deficiencies in growth and rRNA biogenesis that occurs in absence of Yvh1. Here, we report that the interaction between Mrt4 and Yvh1 is also essential for normal glycogen accumulation and mRNA decay as well as the induction of sporulation genes IME2, SPO13 and HOP1. The Mrt4(G68D) could restore the plethora of phenotypes we observed in absence of Yvh1. We found that Yvh1 is not essential for wild-type induction of the transcriptional regulator of these genes, IME1, suggesting that either translation or post-translational modification to activate Ime1 has been compromised. Since a defect in ribosome biogenesis in general can be related to other various defects, the ribosome biogenesis defect caused by absence of Yvh1 might be an indirect cause of observed phenotypes. |
| 巻・号 | 150(1) |
| ページ | 103-11 |
| 公開日 | 2011-7-1 |
| DOI | 10.1093/jb/mvr040 |
| PII | mvr040 |
| PMID | 21474464 |
| MeSH | Amino Acid Sequence DNA-Binding Proteins / genetics Dual-Specificity Phosphatases / genetics* Dual-Specificity Phosphatases / metabolism* Gene Expression Regulation, Developmental Gene Expression Regulation, Fungal Glycogen / genetics Glycogen / metabolism Intracellular Signaling Peptides and Proteins / genetics Meiosis / genetics Molecular Sequence Data Mutation / genetics Nuclear Proteins / genetics* Nuclear Proteins / metabolism Phenotype Protein Processing, Post-Translational Protein Serine-Threonine Kinases / genetics RNA Stability / genetics Ribosomal Proteins / genetics* Ribosomal Proteins / metabolism* Ribosomes / metabolism Saccharomyces cerevisiae / genetics* Saccharomyces cerevisiae / growth & development* Saccharomyces cerevisiae Proteins / genetics* Saccharomyces cerevisiae Proteins / metabolism* Transcription Factors / genetics* Transcription Factors / metabolism |
| IF | 2.476 |
| 引用数 | 4 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| 酵母 | NA |