RRC ID 35259
Author Bekker M, Alexeeva S, Laan W, Sawers G, Teixeira de Mattos J, Hellingwerf K.
Title The ArcBA two-component system of Escherichia coli is regulated by the redox state of both the ubiquinone and the menaquinone pool.
Journal J. Bacteriol.
Abstract ArcBA is a two-component regulatory system of Escherichia coli involved in sensing oxygen availability and the concomitant transcriptional regulation of oxidative and fermentative catabolism. Based on in vitro data, it has been postulated that the redox state of the ubiquinone pool is the determinant for ArcB kinase activity. Here we report on the in vivo regulation of ArcB activation, as determined using a lacZ reporter specifically responsive to phosphorylated ArcA. Our results indicate that upon deletion of a ubiquinone biosynthetic enzyme, regulation of ArcB in the anaerobic-aerobic transition is not affected. In contrast, interference with menaquinone biosynthesis leads to inactivation of ArcB during anaerobic growth; this phenotype is fully rescued by addition of a menaquinone precursor. This clearly demonstrates that the menaquinones play a major role in ArcB activation. ArcB shows a complex pattern of regulation when E. coli is titrated through the entire aerobiosis range; ArcB is activated under anaerobic and subaerobic conditions and is much less active under fully aerobic and microaerobic conditions. Furthermore, there is no correlation between ArcB activation and the redox state of the ubiquinone pool, but there is a restricted correlation between the total cellular ubiquinone content and ArcB activity due to the considerable increase in the size of the ubiquinone pool with increasing degrees of aerobiosis. These results lead to the working hypothesis that the in vivo activity of ArcB in E. coli is modulated by the redox state of the menaquinone pool and that the ubiquinone/ubiquinol ratio in vivo surely is not the only determinant of ArcB activity.
Volume 192(3)
Pages 746-54
Published 2010-2
DOI 10.1128/JB.01156-09
PII JB.01156-09
PMID 19933363
PMC PMC2812447
MeSH Bacterial Outer Membrane Proteins / genetics Bacterial Outer Membrane Proteins / metabolism* Blotting, Western Electrophoretic Mobility Shift Assay Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Gene Expression Regulation, Bacterial Membrane Proteins / genetics Membrane Proteins / metabolism* Models, Genetic Operon / genetics Oxidation-Reduction Phosphorylation Protein Binding / genetics Protein Binding / physiology Protein Kinases / genetics Protein Kinases / metabolism* Repressor Proteins / genetics Repressor Proteins / metabolism* Ubiquinone / metabolism* Vitamin K 2 / metabolism*
IF 3.219
Times Cited 71
WOS Category MICROBIOLOGY
Resource
Prokaryotes E. coli ME9062(BW25113) JW2257-KC JW5713-KC JW2337-KC JW5536-KC ?