| RRC ID |
35273
|
| Author |
Levin-Reisman I, Gefen O, Fridman O, Ronin I, Shwa D, Sheftel H, Balaban NQ.
|
| Title |
Automated imaging with ScanLag reveals previously undetectable bacterial growth phenotypes.
|
| Journal |
Nat Methods
|
| Abstract |
We developed an automated system, ScanLag, that measures in parallel the delay in growth (lag time) and growth rate of thousands of cells. Using ScanLag, we detected small subpopulations of bacteria with dramatically increased lag time upon starvation. By screening a library of Escherichia coli deletion mutants, we achieved two-dimensional mapping of growth characteristics, which showed that ScanLag enables multidimensional screens for quantitative characterization and identification of rare phenotypic variants.
|
| Volume |
7(9)
|
| Pages |
737-9
|
| Published |
2010-9-1
|
| DOI |
10.1038/nmeth.1485
|
| PII |
nmeth.1485
|
| PMID |
20676109
|
| MeSH |
Automation
Escherichia coli / genetics
Escherichia coli / growth & development*
Gene Library
Genetic Variation
Image Processing, Computer-Assisted / methods*
Mutation
Phenotype
|
| IF |
30.822
|
| Times Cited |
67
|
|
WOS Category
|
BIOCHEMICAL RESEARCH METHODS
|
| Resource |
| Prokaryotes E. coli |
Keio collection
the large and medium deletion mutants library |
