| RRC ID |
3530
|
| 著者 |
Pan X, Ou G, Civelekoglu-Scholey G, Blacque OE, Endres NF, Tao L, Mogilner A, Leroux MR, Vale RD, Scholey JM.
|
| タイトル |
Mechanism of transport of IFT particles in C. elegans cilia by the concerted action of kinesin-II and OSM-3 motors.
|
| ジャーナル |
J Cell Biol
|
| Abstract |
The assembly and function of cilia on Caenorhabditis elegans neurons depends on the action of two kinesin-2 motors, heterotrimeric kinesin-II and homodimeric OSM-3-kinesin, which cooperate to move the same intraflagellar transport (IFT) particles along microtubule (MT) doublets. Using competitive in vitro MT gliding assays, we show that purified kinesin-II and OSM-3 cooperate to generate movement similar to that seen along the cilium in the absence of any additional regulatory factors. Quantitative modeling suggests that this could reflect an alternating action mechanism, in which the motors take turns to move along MTs, or a mechanical competition, in which the motors function in a concerted fashion to move along MTs with the slow motor exerting drag on the fast motor and vice versa. In vivo transport assays performed in Bardet-Biedl syndrome (BBS) protein and IFT motor mutants favor a mechanical competition model for motor coordination in which the IFT motors exert a BBS protein-dependent tension on IFT particles, which controls the IFT pathway that builds the cilium foundation.
|
| 巻・号 |
174(7)
|
| ページ |
1035-45
|
| 公開日 |
2006-9-25
|
| DOI |
10.1083/jcb.200606003
|
| PII |
jcb.200606003
|
| PMID |
17000880
|
| PMC |
PMC2064394
|
| MeSH |
Animals
Animals, Genetically Modified
Biological Transport
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Cells, Cultured
Cilia / metabolism
Cilia / physiology*
Kinesins / genetics
Kinesins / metabolism*
Models, Biological
Molecular Motor Proteins / metabolism*
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Sea Urchins
|
| IF |
8.811
|
| 引用数 |
132
|
|
WOS 分野
|
CELL BIOLOGY
|
| リソース情報 |
| 線虫 |
tm324 |
