論文 - 詳細
| RRC ID | 35317 |
|---|---|
| 著者 | Tashiro Y, Fukutomi H, Terakubo K, Saito K, Umeno D. |
| タイトル | A nucleoside kinase as a dual selector for genetic switches and circuits. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
The development of genetic switches and their integrated forms (genetic circuits) with desired specifications/functions is key for success in synthetic biology. Due to the difficulty in rational design, genetic switches and circuits with desirable specifications are mostly obtained by directed evolution. Based on a virus-derived nucleotide kinase as a single-gene dual selector, we constructed a robust, efficient and stringent selection system for genetic switches. This method exhibited unprecedented enrichment efficacy (>30,000-fold) of functional switches from non-functional ones in a single selection cycle. In addition, negative (OFF) selection was exceptionally stringent, allowing the rapid and efficient selection of non-leaky from leaky circuits. |
| 巻・号 | 39(3) |
| ページ | e12 |
| 公開日 | 2011-2-1 |
| DOI | 10.1093/nar/gkq1070 |
| PII | gkq1070 |
| PMID | 21062820 |
| PMC | PMC3035434 |
| MeSH | Gene Regulatory Networks* Genetic Engineering / methods* Models, Genetic Simplexvirus / enzymology Thymidine Kinase / metabolism* |
| IF | 11.502 |
| 引用数 | 23 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | JW1226-KC |