RRC ID |
35317
|
著者 |
Tashiro Y, Fukutomi H, Terakubo K, Saito K, Umeno D.
|
タイトル |
A nucleoside kinase as a dual selector for genetic switches and circuits.
|
ジャーナル |
Nucleic Acids Res
|
Abstract |
The development of genetic switches and their integrated forms (genetic circuits) with desired specifications/functions is key for success in synthetic biology. Due to the difficulty in rational design, genetic switches and circuits with desirable specifications are mostly obtained by directed evolution. Based on a virus-derived nucleotide kinase as a single-gene dual selector, we constructed a robust, efficient and stringent selection system for genetic switches. This method exhibited unprecedented enrichment efficacy (>30,000-fold) of functional switches from non-functional ones in a single selection cycle. In addition, negative (OFF) selection was exceptionally stringent, allowing the rapid and efficient selection of non-leaky from leaky circuits.
|
巻・号 |
39(3)
|
ページ |
e12
|
公開日 |
2011-2-1
|
DOI |
10.1093/nar/gkq1070
|
PII |
gkq1070
|
PMID |
21062820
|
PMC |
PMC3035434
|
MeSH |
Gene Regulatory Networks*
Genetic Engineering / methods*
Models, Genetic
Simplexvirus / enzymology
Thymidine Kinase / metabolism*
|
IF |
11.502
|
引用数 |
23
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
原核生物(大腸菌) |
JW1226-KC |