論文 - 詳細
| RRC ID | 3539 |
|---|---|
| 著者 | Robert V, Bessereau JL. |
| タイトル | Targeted engineering of the Caenorhabditis elegans genome following Mos1-triggered chromosomal breaks. |
| ジャーナル | EMBO J |
| Abstract |
The Drosophila element Mos1 is a class II transposon, which moves by a 'cut-and-paste' mechanism and can be experimentally mobilized in the Caenorhabditis elegans germ line. Here, we triggered the excision of identified Mos1 insertions to create chromosomal breaks at given sites and further manipulate the broken loci. Double-strand break (DSB) repair could be achieved by gene conversion using a transgene containing sequences homologous to the broken chromosomal region as a repair template. Consequently, mutations engineered in the transgene could be copied to a specific locus at high frequency. This pathway was further characterized to develop an efficient tool--called MosTIC--to manipulate the C. elegans genome. Analysis of DSB repair during MosTIC experiments demonstrated that DSBs could also be sealed by end-joining in the germ line, independently from the evolutionarily conserved Ku80 and ligase IV factors. In conjunction with a publicly available Mos1 insertion library currently being generated, MosTIC will provide a general tool to customize the C. elegans genome. |
| 巻・号 | 26(1) |
| ページ | 170-83 |
| 公開日 | 2007-1-10 |
| DOI | 10.1038/sj.emboj.7601463 |
| PII | 7601463 |
| PMID | 17159906 |
| PMC | PMC1782371 |
| MeSH | Animals Base Sequence Caenorhabditis elegans / genetics* Caenorhabditis elegans / physiology Computational Biology DNA Breaks, Double-Stranded* DNA-Binding Proteins / genetics* Drosophila melanogaster / metabolism Genetic Techniques* Genome* Models, Genetic Molecular Sequence Data Mutagenesis Plasmids / metabolism Recombination, Genetic Transgenes* Transposases / genetics* |
| IF | 9.889 |
| 引用数 | 70 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm750 |