RRC ID 3539
Author Robert V, Bessereau JL.
Title Targeted engineering of the Caenorhabditis elegans genome following Mos1-triggered chromosomal breaks.
Journal EMBO J.
Abstract The Drosophila element Mos1 is a class II transposon, which moves by a 'cut-and-paste' mechanism and can be experimentally mobilized in the Caenorhabditis elegans germ line. Here, we triggered the excision of identified Mos1 insertions to create chromosomal breaks at given sites and further manipulate the broken loci. Double-strand break (DSB) repair could be achieved by gene conversion using a transgene containing sequences homologous to the broken chromosomal region as a repair template. Consequently, mutations engineered in the transgene could be copied to a specific locus at high frequency. This pathway was further characterized to develop an efficient tool--called MosTIC--to manipulate the C. elegans genome. Analysis of DSB repair during MosTIC experiments demonstrated that DSBs could also be sealed by end-joining in the germ line, independently from the evolutionarily conserved Ku80 and ligase IV factors. In conjunction with a publicly available Mos1 insertion library currently being generated, MosTIC will provide a general tool to customize the C. elegans genome.
Volume 26(1)
Pages 170-83
Published 2007-1-10
DOI 10.1038/sj.emboj.7601463
PII 7601463
PMID 17159906
PMC PMC1782371
MeSH Animals Base Sequence Caenorhabditis elegans / genetics* Caenorhabditis elegans / physiology Computational Biology DNA Breaks, Double-Stranded* DNA-Binding Proteins / genetics* Drosophila melanogaster / metabolism Genetic Techniques* Genome* Models, Genetic Molecular Sequence Data Mutagenesis Plasmids / metabolism Recombination, Genetic Transgenes* Transposases / genetics*
IF 10.557
Times Cited 64
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
C.elegans tm750