RRC ID 35523
著者 Jang S, Sandler SJ, Harshey RM.
タイトル Mu insertions are repaired by the double-strand break repair pathway of Escherichia coli.
ジャーナル PLoS Genet
Abstract Mu is both a transposable element and a temperate bacteriophage. During lytic growth, it amplifies its genome by replicative transposition. During infection, it integrates into the Escherichia coli chromosome through a mechanism not requiring extensive DNA replication. In the latter pathway, the transposition intermediate is repaired by transposase-mediated resecting of the 5' flaps attached to the ends of the incoming Mu genome, followed by filling the remaining 5 bp gaps at each end of the Mu insertion. It is widely assumed that the gaps are repaired by a gap-filling host polymerase. Using the E. coli Keio Collection to screen for mutants defective in recovery of stable Mu insertions, we show in this study that the gaps are repaired by the machinery responsible for the repair of double-strand breaks in E. coli-the replication restart proteins PriA-DnaT and homologous recombination proteins RecABC. We discuss alternate models for recombinational repair of the Mu gaps.
巻・号 8(4)
ページ e1002642
公開日 2012-1-1
DOI 10.1371/journal.pgen.1002642
PII PGENETICS-D-11-01986
PMID 22511883
PMC PMC3325207
MeSH Bacteriophage mu* / genetics Bacteriophage mu* / growth & development DNA Breaks, Double-Stranded DNA Helicases / genetics DNA Helicases / metabolism DNA Repair* / genetics DNA Transposable Elements / genetics* DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Escherichia coli* / genetics Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism Genome Homologous Recombination / genetics* Mutagenesis, Insertional Mutation Transposases / metabolism
IF 5.175
引用数 0
リソース情報
原核生物(大腸菌) Keio collection