RRC ID 356
Author Takahashi A, Takano-Shimizu T.
Title A high-frequency null mutant of an odorant-binding protein gene, Obp57e, in Drosophila melanogaster.
Journal Genetics
Abstract We have found a null mutant of an odorant-binding protein, Obp57e, in Drosophila melanogaster. This frameshift mutation, which is a 10-bp deletion in the coding region, is at a high frequency in the Kyoto population and is also present in Taiwan and Africa. We have sequenced a 1.5-kb region including the tandemly duplicated gene, Obp57d, from 16 inbred lines sampled in Kyoto, Japan. The analyses showed a peak of nucleotide diversity and strong linkage disequilibrium around this mutation. This pattern suggests an elevated mutation rate or an influence of balancing selection in this region. The level of nucleotide divergence between D. melanogaster and D. simulans does not support the former possibility. Thus, this presence/absence polymorphism may be due to balancing selection, which takes advantage of the relatively weak functional constraint in members of a large gene family. In addition, the Obp57d gene region showed an excess of high-frequency-derived mutants that is consistent with a pattern predicted under positive natural selection.
Volume 170(2)
Pages 709-18
Published 2005-6
DOI 10.1534/genetics.104.036483
PII genetics.104.036483
PMID 15802511
PMC PMC1450408
MeSH Alleles Animals DNA / metabolism DNA Mutational Analysis Drosophila Drosophila Proteins / genetics* Drosophila Proteins / physiology Drosophila melanogaster / genetics* Female Frameshift Mutation Genotype Heterozygote Linkage Disequilibrium Male Models, Genetic Molecular Sequence Data Mutation* Physical Chromosome Mapping Polymorphism, Genetic Receptors, Odorant / genetics* Receptors, Odorant / physiology Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis, DNA
IF 3.564
Times Cited 11