RRC ID 35694
著者 Daimon E, Shibukawa Y, Wada Y.
タイトル Calponin 3 regulates stress fiber formation in dermal fibroblasts during wound healing.
ジャーナル Arch Dermatol Res
Abstract Skin wound healing is an intricate process involving various cell types and molecules. In granulation tissue, fibroblasts proliferate and differentiate into myofibroblasts and generate mechanical tension for wound closure and contraction. Actin stress fibers formed in these cells, especially those containing α-smooth muscle actin (α-SMA), are the central machinery for contractile force generation. In the present study, calponin 3 (CNN3), which has a phosphorylation-dependent actin-binding property, was identified in the molecular mechanism underlying stress fiber formation. CNN3 was expressed by fibroblasts/myofibroblasts in the proliferation phase of wound healing, and was associated with α-SMA in stress fibers formed by cultured dermal fibroblasts. CNN3 expression was post-transcriptionally regulated by tension, as demonstrated by disruption of actin filament organization under floating culture or blebbistatin treatment. CNN3 knockdown in primary fibroblasts impaired stress fiber formation, resulting in a phenotype of decreased cellular dynamics such as cell motility and contractile ability. These findings indicate that CNN3 participates in actin stress fiber remodeling, which is required for cell motility and contraction of dermal fibroblasts in the wound healing process.
巻・号 305(7)
ページ 571-84
公開日 2013-9-1
DOI 10.1007/s00403-013-1343-8
PMID 23545751
MeSH Actin Cytoskeleton / drug effects Actin Cytoskeleton / pathology Actins / genetics Actins / metabolism Animals Calcium-Binding Proteins / genetics Calcium-Binding Proteins / metabolism* Cell Movement / drug effects Cell Movement / genetics Cells, Cultured Dermis / pathology Fibroblasts / drug effects Fibroblasts / metabolism* Fibroblasts / pathology Granulation Tissue / pathology Heterocyclic Compounds, 4 or More Rings / pharmacology Mice Microfilament Proteins / genetics Microfilament Proteins / metabolism* Muscle Contraction / drug effects Muscle Contraction / genetics Myofibroblasts / drug effects Myofibroblasts / metabolism Myofibroblasts / pathology RNA Processing, Post-Transcriptional RNA, Small Interfering / genetics Stress Fibers / metabolism* Stress Fibers / ultrastructure Wound Healing / physiology*
IF 2.339
引用数 31
WOS 分野 DERMATOLOGY
リソース情報
遺伝子材料 pCAG-HIVgp (RDB04394) pCMV-VSV-G-RSV-Rev (RDB04393).