論文 - 詳細
| RRC ID | 3574 |
|---|---|
| 著者 | Gomez TA, Banfield KL, Trogler DM, Clarke SG. |
| タイトル | The L-isoaspartyl-O-methyltransferase in Caenorhabditis elegans larval longevity and autophagy. |
| ジャーナル | Dev Biol |
| Abstract |
The protein L-isoaspartyl-O-methyltransferase, coded by the pcm-1 gene in Caenorhabditis elegans, participates in the repair of age-damaged proteins. We tested the ability of pcm-1-deficient nematodes to survive starvation stress as developmentally-arrested L1 larvae. We found that pcm-1 mutant L1 larvae do not survive as well as wild-type L1 larvae when incubated in M9 medium without nutrients. We then tested whether the starved L1 larvae could continue development when allowed access to food in a recovery assay. A loss of recovery ability with age was observed for all larvae, with little or no difference between the pcm-1 mutant and wild-type N2 larvae. Interestingly, when L1 larvae were starved in cholesterol-containing S medium or M9 medium supplemented with cholesterol, the survival rates of both mutant and wild-type animals nearly doubles, with pcm-1 larvae again faring more poorly than N2 larvae. Furthermore, L1 larvae cultured in these cholesterol-containing media show an increase in Sudan Black staining over animals cultured in M9 medium. The longevity defects of pcm-1 mutants previously seen in dauer larvae and here in L1 larvae suggest a defect in the ability of pcm-1 mutants to recycle and reuse old cellular components in pathways such as autophagy. Using an autophagosomal marker, we found evidence suggesting that the pcm-1 mutation may inhibit autophagy during dauer formation, suggesting that the absence of protein repair may also interfere with protein degradation pathways. |
| 巻・号 | 303(2) |
| ページ | 493-500 |
| 公開日 | 2007-3-15 |
| DOI | 10.1016/j.ydbio.2006.11.023 |
| PII | S0012-1606(06)01378-9 |
| PMID | 17187774 |
| PMC | PMC1868680 |
| MeSH | Animals Autophagy / genetics Autophagy / physiology* Caenorhabditis elegans / enzymology Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / physiology* Cell Cycle Proteins / genetics Cell Cycle Proteins / physiology* Cholesterol / metabolism Genes, Helminth Larva / enzymology Longevity / genetics Longevity / physiology* Methyltransferases / genetics Methyltransferases / physiology* Mutation Starvation |
| IF | 2.896 |
| 引用数 | 19 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 線虫 | tm363 |