| RRC ID |
3581
|
| Author |
Haynes CM, Petrova K, Benedetti C, Yang Y, Ron D.
|
| Title |
ClpP mediates activation of a mitochondrial unfolded protein response in C. elegans.
|
| Journal |
Dev Cell
|
| Abstract |
The cellular response to unfolded and misfolded proteins in the mitochondrial matrix is poorly understood. Here, we report on a genome-wide RNAi-based screen for genes that signal the mitochondrial unfolded protein response (UPR(mt)) in C. elegans. Unfolded protein stress in the mitochondria correlates with complex formation between a homeodomain-containing transcription factor DVE-1 and the small ubiquitin-like protein UBL-5, both of which are encoded by genes required for signaling the UPR(mt). Activation of the UPR(mt) correlates temporally and spatially with nuclear redistribution of DVE-1 and with its enhanced binding to the promoters of mitochondrial chaperone genes. These events and the downstream UPR(mt) are attenuated in animals with reduced activity of clpp-1, which encodes a mitochondrial matrix protease homologous to bacterial ClpP. As ClpP is known to function in the bacterial heat-shock response, our findings suggest that eukaryotes utilize component(s) from the protomitochondrial symbiont to signal the UPR(mt).
|
| Volume |
13(4)
|
| Pages |
467-80
|
| Published |
2007-10-1
|
| DOI |
10.1016/j.devcel.2007.07.016
|
| PII |
S1534-5807(07)00277-8
|
| PMID |
17925224
|
| MeSH |
Animals
Animals, Genetically Modified
Caenorhabditis elegans / embryology
Caenorhabditis elegans / genetics
Caenorhabditis elegans / metabolism*
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Cell Nucleus / metabolism
Embryo, Nonmammalian
Endopeptidase Clp / genetics
Endopeptidase Clp / metabolism*
Escherichia coli Proteins / genetics
Genome, Helminth
Mitochondria / metabolism*
Molecular Chaperones / genetics
Molecular Chaperones / metabolism
Mutation
Promoter Regions, Genetic
Protein Folding*
Signal Transduction
Transcription Factors / metabolism*
Ubiquitins / genetics
Ubiquitins / metabolism*
|
| IF |
10.092
|
| Times Cited |
295
|
|
WOS Category
|
DEVELOPMENTAL BIOLOGY
CELL BIOLOGY
|
| Resource |
| C.elegans |
tm259 |
