RRC ID 3582
著者 Kobayashi T, Gengyo-Ando K, Ishihara T, Katsura I, Mitani S.
タイトル IFT-81 and IFT-74 are required for intraflagellar transport in C. elegans.
ジャーナル Genes Cells
Abstract Intraflagellar transport (IFT) is essential machinery for biogenesis and maintenance of cilia in many eukaryotic and prokaryotic cells. A large number of polypeptides are known to be involved in IFT, but the physiological role of each component is not fully elucidated. Here, we identified a C. elegans orthologue of a Chlamydomonas reinhardtii IFT component, IFT-81, and found that its loss-of-function mutants show an unusual behavioral property and small body size. IFT-81 is expressed in sensory neurons, and localized at the base of cilia. The similar phenotypes with ift-81 mutants were also observed in several IFT mutants, suggesting these defects are caused by inability of IFT. We also demonstrated that IFT-81 interacts and co-localizes with IFT-74, which is another putative component of IFT. The ift-74 loss-of-function mutants showed phenocopies with ift-81 mutants, suggesting IFT-81 and IFT-74 play comparable functions. Moreover, ift-81 and ift-74 mutants similarly exhibited weak anomalies in cilia formation and obvious disruptions of transport in mature cilia. Thus, we conclude that IFT-81 and IFT-74 coordinately act in IFT in C. elegans sensory cilia.
巻・号 12(5)
ページ 593-602
公開日 2007-5-1
DOI 10.1111/j.1365-2443.2007.01076.x
PII GTC1076
PMID 17535250
MeSH Animals Animals, Genetically Modified Biological Transport, Active Caenorhabditis elegans / genetics Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / physiology* Carrier Proteins / genetics Carrier Proteins / physiology* Chemotaxis Cilia / physiology Flagella / physiology* Genes, Helminth Mutation Neurons, Afferent / physiology Phenotype
IF 1.655
引用数 20
WOS 分野 GENETICS & HEREDITY CELL BIOLOGY
リソース情報
線虫 tm2355 tm2356 tm2389 tm2394 tm2397