論文 - 詳細
| RRC ID | 3582 |
|---|---|
| 著者 | Kobayashi T, Gengyo-Ando K, Ishihara T, Katsura I, Mitani S. |
| タイトル | IFT-81 and IFT-74 are required for intraflagellar transport in C. elegans. |
| ジャーナル | Genes Cells |
| Abstract |
Intraflagellar transport (IFT) is essential machinery for biogenesis and maintenance of cilia in many eukaryotic and prokaryotic cells. A large number of polypeptides are known to be involved in IFT, but the physiological role of each component is not fully elucidated. Here, we identified a C. elegans orthologue of a Chlamydomonas reinhardtii IFT component, IFT-81, and found that its loss-of-function mutants show an unusual behavioral property and small body size. IFT-81 is expressed in sensory neurons, and localized at the base of cilia. The similar phenotypes with ift-81 mutants were also observed in several IFT mutants, suggesting these defects are caused by inability of IFT. We also demonstrated that IFT-81 interacts and co-localizes with IFT-74, which is another putative component of IFT. The ift-74 loss-of-function mutants showed phenocopies with ift-81 mutants, suggesting IFT-81 and IFT-74 play comparable functions. Moreover, ift-81 and ift-74 mutants similarly exhibited weak anomalies in cilia formation and obvious disruptions of transport in mature cilia. Thus, we conclude that IFT-81 and IFT-74 coordinately act in IFT in C. elegans sensory cilia. |
| 巻・号 | 12(5) |
| ページ | 593-602 |
| 公開日 | 2007-5-1 |
| DOI | 10.1111/j.1365-2443.2007.01076.x |
| PII | GTC1076 |
| PMID | 17535250 |
| MeSH | Animals Animals, Genetically Modified Biological Transport, Active Caenorhabditis elegans / genetics Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / physiology* Carrier Proteins / genetics Carrier Proteins / physiology* Chemotaxis Cilia / physiology Flagella / physiology* Genes, Helminth Mutation Neurons, Afferent / physiology Phenotype |
| IF | 1.655 |
| 引用数 | 20 |
| WOS 分野 | GENETICS & HEREDITY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 線虫 | tm2355 tm2356 tm2389 tm2394 tm2397 |