Reference - Detail
| RRC ID | 36048 |
|---|---|
| Author | Kuse Y, Tsuruma K, Sugitani S, Izawa H, Ohno Y, Shimazawa M, Hara H. |
| Title | Progranulin promotes the retinal precursor cell proliferation and the photoreceptor differentiation in the mouse retina. |
| Journal | Sci Rep |
| Abstract |
Progranulin (PGRN) is a secreted growth factor associated with embryo development, tissue repair, and inflammation. In a previous study, we showed that adipose-derived stem cell-conditioned medium (ASC-CM) is rich in PGRN. In the present study, we investigated whether PGRN is associated with retinal regeneration in the mammalian retina. We evaluated the effect of ASC-CM using the N-methyl-N-nitrosourea-induced retinal damage model in mice. ASC-CM promoted the differentiation of photoreceptor cells following retinal damage. PGRN increased the number of BrdU(+) cells in the outer nuclear layer following retinal damage some of which were Rx (retinal precursor cell marker) positive. PGRN also increased the number of rhodopsin(+) photoreceptor cells in primary retinal cell cultures. SU11274, a hepatocyte growth factor (HGF) receptor inhibitor, attenuated the increase. These findings suggest that PGRN may affect the differentiation of retinal precursor cells to photoreceptor cells through the HGF receptor signaling pathway. |
| Volume | 6 |
| Pages | 23811 |
| Published | 2016-3-31 |
| DOI | 10.1038/srep23811 |
| PII | srep23811 |
| PMID | 27030285 |
| PMC | PMC4814875 |
| MeSH | Adipocytes / cytology Adipocytes / metabolism Animals Cell Differentiation Culture Media, Conditioned / chemistry Culture Media, Conditioned / pharmacology* Eye Proteins / genetics Eye Proteins / metabolism Female Gene Expression Regulation Granulins Hepatocyte Growth Factor / antagonists & inhibitors Hepatocyte Growth Factor / genetics* Hepatocyte Growth Factor / metabolism Homeodomain Proteins / genetics Homeodomain Proteins / metabolism Indoles / pharmacology Intercellular Signaling Peptides and Proteins / genetics Intercellular Signaling Peptides and Proteins / metabolism Intercellular Signaling Peptides and Proteins / pharmacology* Male Methylnitrosourea / pharmacology Mice Mice, Inbred C57BL Photoreceptor Cells, Vertebrate / cytology Photoreceptor Cells, Vertebrate / drug effects* Photoreceptor Cells, Vertebrate / metabolism Piperazines / pharmacology Primary Cell Culture Progranulins Regeneration / drug effects* Regeneration / genetics Retina / drug effects Retina / injuries Retina / metabolism Rhodopsin / genetics Rhodopsin / metabolism Signal Transduction Stem Cells / cytology Stem Cells / metabolism Sulfonamides / pharmacology |
| IF | 3.998 |
| Times Cited | 11 |
| WOS Category | NEUROSCIENCES |
| Resource | |
| Mice | RBRC02370 |