| RRC ID |
3611
|
| 著者 |
Ou G, Koga M, Blacque OE, Murayama T, Ohshima Y, Schafer JC, Li C, Yoder BK, Leroux MR, Scholey JM.
|
| タイトル |
Sensory ciliogenesis in Caenorhabditis elegans: assignment of IFT components into distinct modules based on transport and phenotypic profiles.
|
| ジャーナル |
Mol Biol Cell
|
| Abstract |
Sensory cilium biogenesis within Caenorhabditis elegans neurons depends on the kinesin-2-dependent intraflagellar transport (IFT) of ciliary precursors associated with IFT particles to the axoneme tip. Here we analyzed the molecular organization of the IFT machinery by comparing the in vivo transport and phenotypic profiles of multiple proteins involved in IFT and ciliogenesis. Based on their motility in wild-type and bbs (Bardet-Biedl syndrome) mutants, IFT proteins were classified into groups with similar transport profiles that we refer to as "modules." We also analyzed the distribution and transport of fluorescent IFT particles in multiple known ciliary mutants and 49 new ciliary mutants. Most of the latter mutants were snip-SNP mapped and one, namely dyf-14(ks69), was cloned and found to encode a conserved protein essential for ciliogenesis. The products of these ciliogenesis genes could also be assigned to the aforementioned set of modules or to specific aspects of ciliogenesis, based on IFT particle dynamics and ciliary mutant phenotypes. Although binding assays would be required to confirm direct physical interactions, the results are consistent with the hypothesis that the C. elegans IFT machinery has a modular design, consisting of modules IFT-subcomplex A, IFT-subcomplex B, and a BBS protein complex, in addition to motor and cargo modules, with each module contributing to distinct functional aspects of IFT or ciliogenesis.
|
| 巻・号 |
18(5)
|
| ページ |
1554-69
|
| 公開日 |
2007-5-1
|
| DOI |
10.1091/mbc.e06-09-0805
|
| PII |
E06-09-0805
|
| PMID |
17314406
|
| PMC |
PMC1855012
|
| MeSH |
Animals
Animals, Genetically Modified
Biological Transport, Active
Caenorhabditis elegans / genetics
Caenorhabditis elegans / physiology*
Caenorhabditis elegans / ultrastructure
Caenorhabditis elegans Proteins / chemistry
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / physiology
Cilia / physiology*
Cilia / ultrastructure
Genes, Helminth
Green Fluorescent Proteins / genetics
Microscopy, Fluorescence
Models, Biological
Mutation
Phenotype
Recombinant Fusion Proteins / genetics
|
| IF |
3.791
|
| 引用数 |
93
|
|
WOS 分野
|
CELL BIOLOGY
|
| リソース情報 |
| 線虫 |
tm2085 |
